Identification of Superoxide Dismutase Genes and Expression Analysis Under Cold Stress in Sisal
The sisal is an important fiber crop in tropical areas,which is easily threatened by cold stress,resulting in reduced production.Superoxide dismutase(SOD)is an important protective enzyme that plays an important role in plant response to stress conditions including cold stress.Previous studies have preliminarily confirmed the important role of SOD under cold stress in sisal,but few are known about their gene sequences and molecular regulatory mechanisms in sisal.In this study,five SOD genes according to the published sisal transcriptome database,and genetic evolution analysis showed the relatively conserved evolution of SOD genes in different plant species.The real-time quantitative PCR results indicated that the expression of five genes increased and then decreased under cold stress.Their expression patterns were also positively correlated with SOD activity(P<0.05).Among these,two CSD genes and one FSD gene were significantly positive correlated with SOD activity(P<0.01),which indicated their important roles in response to cold stress.This study identified the SOD genes in sisal and preliminarily clarified their expression patterns in response to cold stress,providing an important basis for further researches related to the mechanisms of cold stress response in sisal.