Research on Tissue Culture and Rapid Propagation Technology of Phalaenopsis'Zhengnong Hongyu'
In order to screen a set of simple and efficient tissue culture and rapid propagation technology system of P.'Zhengnong Hongyu',and meet the market demand for large-scale production,the axillary buds of the pedicel of P.'Zhengnong Hongyu'were used as explants.Pedicel axillary bud induction,proliferation of clustered buds,robust seedling and rooting cultures were carried out sequentially,and the influencing factors such as basic medium,plant growth regulators and organic additives were set as three treatments for orthogonal tests,so as to explore the suitable medium formulations for different growth stages.The results showed that pedicel axillary buds were better induced on the medium of"Hyponex NO.1"2 g/L+"Centrum"1 capsule/L+inositol 125 mg/L+6-BA 5.0 mg/L+IBA 0.5 mg/L+mashed potato 1000 g/L+sucrose 25 g/L;the shoots with the terminal buds cut off grew better on the medium of"Hyponex NO.1"2 g/L+"Centrum"1 capsule/L+inositol 125 mg/L+6-BA 2.5mg/L+IBA 0.5 mg/L+mashed potato 1000 g/L+sucrose 20 g/L,and the proliferation rate reached 4 times;the proliferated seedlings could reach uniformity in the seedling-strengthening medium of"Hyponex NO.1"2 g/L+"Centrum"1 capsule/L+inositol 125 mg/L+IBA 0.5 mg/L+NAA 0.7 mg/L+mashed potato 1000 g/L+sucrose 25 g/L;the rootless seedlings rooted best on the medium of"Hyponex NO.1"2 g/L+"Centrum"1 capsule/L+inositol 125 mg/L+IBA 0.5 mg/L+mashed banana 60 g/L+mashed potato 60 g/L+AC 1 g/L+sucrose 20 g/L.The aim was to lay a technical foundation for large-scale rapid propagation of P.'Zhengnonghongyu'.
Phalaenopsis'Zhengnong Hongyu'pedicel axillary budtissue culture and rapid propagation
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