Identification of sulfakinin and its receptor gene in Aedes aegypti and transcriptomic changes in genes knockdown strains
Objective By exploring the function of sulfakinin(SK)and sulfakinin receptor(SKR)of Aedes aegypti,it laid a certain experimental basis and theoretical basis for the research and development of new insecticides targeting neuropeptides and their receptors.Methods This study investigated the roles of SK and its receptor gene in Ae.aegypti using bioinformatics analysis and Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)/Cas9 knockout technology.Subsequently,RNA interference technology was employed to suppress the expression of SK or its receptor in adult mosquitoes.Lastly,transcriptome sequencing technology was utilized to identify and analyze differentially expressed genes between the interference group and the control group in order to gain insights into their functions.Results It was found that there is only one SK receptor in Ae.aegypti.In addition,during the construction of mutant strains of Ae.aegypti SK and its receptor gene,it was found that only 2% of the G0 generation mutant strains mutated to form chimeras,with a large number of male chimeras dying,and only 14% of female chimeras being able to lay eggs,ultimately resulting in no effective G1 generation mutants.Transcriptome data showed,compared to the control group,181 genes were significantly differentially expressed after interfering with the SK gene,with 62 genes significantly upregulated and 119 genes significantly downregulated.In addition,after interference with the sulfakinin receptor,110 genes exhibited significant differential expression,including 20 upregulated and 90 downregulated genes.Cross-analysis of the two datasets identified 46 genes with significant expression changes after interference with sulfakinin or its receptor,with only 4 genes upregulated and the remaining 42 genes significantly downregulated,and the differentially expressed genes were mainly enriched in the metabolic pathway,endocrine system,and digestive system.Conclusions The SK and its receptor gene are highly conserved and may primarily play roles in regulating the energy metabolism and digestion functions in Ae.aegypti,thus playing an important role in regulating insect growth and development.
NETL
NSTL
万方数据
维普
