Evaluation value of miRNA-21 and miRNA-431 in the development and prognosis of pulmonary tuberculosis
Objective To evaluate the value of bronchoscopy specimens'microRNA-21(miRNA-21)and microRNA-431(miRNA-431)in the development and prognosis of pulmonary tuberculosis.Methods A total of 43 confirmed pulmonary tuberculosis patients admitted to Shaanxi Provincial Tuberculosis Prevention Hospital from May 2022 to July 2023 were included as the study group,and 43 healthy volunteers during the same period were included as the control group.In the study group,the severity of lung lesions was classified as mild in 14 cases,moderate in 19 cases,and severe in 10 cases.According to the prognosis,patients were divided into poor prognosis and good prognosis groups.The miRNA-21 and miRNA-431 levels of patients with different disease severity and prognosis in the study group and the control group were compared,and the factors affecting the development and prognosis of tuberculosis were analyzed by logistic multivariate analysis,and a prediction model for the development and prognosis of tuberculosis was established.Receiver operating characteristic(ROC)curve analysis was performed to evaluate the predictive value of the model.Results The levels of miRNA-21 and miRNA-431 in the study group were significantly higher than those in the control group,and the difference between groups was statistically significant(P＜0.05).In the study group,the levels of miRNA-21 and miRNA-431 were positively correlated with the severity of the disease,and the levels in severe pulmonary tuberculosis patients were significantly higher than those in mild and moderate pulmonary tuberculosis patients,with statistically significant differences between the groups(P＜0.05).In the study group,the levels of miRNA-21 and miRNA-431 in patients with pulmonary tuberculosis with poor prognosis were significantly higher than those in patients with pulmonary tuberculosis with good prognosis,with statistically significant differences between the groups(P＜0.05).Using miRNA-21 and miRNA-431 expression as independent variables,and the development and prognosis of pulmonary tuberculosis as dependent variables,logistic regression analysis was performed.The results showed that the high expression of miRNA-21 and miRNA-431 were both influential factors for the development and prognosis of pulmonary tuberculosis(P＜0.05).The ROC curve analysis showed that the optimal cutoff value of the prediction model was 0.0895,and the area under the curve(AUC)of miRNA-21,miRNA-431 and combined were 0.614(0.651-0.732),0.671(0.686-0.811),and 0.891(0.787-0.993).Conclusions The high expression levels of miRNA-21 and miRNA-431 in patients with tuberculosis are closely related to the development of the disease and can predict the prognosis of patients with tuberculosis to a certain extent.The comprehensive application value of combined diagnosis is high,and it is recommended to promote clinical use.
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