Research on the impact of type Ⅱ dengue virus infection on the survival of Aedes albopictus
Objective To investigate the effect of type Ⅱ dengue virus infection on the survival rate of Aedes albopictus,to provide basic data for the prevention and control of dengue fever.Methods The Aedes albopictus C6/36 cells were cultured to amplify and harvest type Ⅱ dengue virus.After emergence,the lab-domesticated Aedes albopictus was fed daily with freshly prepared 10％glucose water under 28℃ and humidity ≥70％,and used for infection experiment at 3-5 days of age.The day before infection,male mosquitoes were selected out,and the female mosquitoes were divided into infected and uninfected groups,deprived of food and water.When infected,they respectively were fed blood meals containing or without dengue virus type Ⅱ(the blood meal of the uninfected group was the same as the infected group's,minus the presence of Type Ⅱ dengue virus),and the saturated blood mosquitoes were fed with 10％glucose water.The survival of mosquitoes in two groups was observed and recorded daily.On the 14th day after blood feeding,the nucleic acid of dengue virus type Ⅱ of Aedes albopictus was detected by fluorescence quantitative PCR,and the infection rate was calculated.The survival rates of infected and uninfected Aedes albopictus were calculated and compared.The survival curves of the two groups were plotted using survival analysis and the log-rank test was performed.Results A total of 99 infected saturated blood Aedes albopictus were reared in the infected group,and 83 in the uninfected group.By the 14th day after infection,31 and 15 mosquitoes had died in the infected and uninfected groups respectively,with mortality rates of 31.31％(31/99)and 18.07％(15/83),respectively,indicating a higher mortality rate in the infected group.The log-rank test showed that the survival curve of the infected group of Aedes albopictus was lower than that of the uninfected group(x2=4.121 9,P＜0.05).The infection rate of Aedes albopictus detected by fluorescence quantitative PCR was 77.94％(53/68).Conclusion Type Ⅱ dengue virus infection can reduce the survival rate of Aedes albopictus.
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