Diagnosis,treatment,and epidemiological investigation of a case of fasciolosis in Laibin,Guangxi
This article reported the diagnosis and treatment of a case of fascioliasis as well as an epidemiological investigation conducted in Laibin City,Guangxi,China.The patient came to the hospital for bellyache.Based on the patient's main symptoms,physical signs,clinical presentation,and laboratory examination(abdominal ultrasound scan and CT),fasciola eggs were detected through the examination of stool specimens using the sedimentation method.Fasciola egg DNA was extracted,and after amplification,sequencing,and comparison of the ITS2 gene,a homology of 99.2％with Fasciola gigantica ITS2 sequences in the NCBI database confirmed the diagnosis of Fasciola gigantica infection.After triclabendazole treatment,the patient's symptoms disappeared,and stool examination yielded negative results for Fasciola eggs.A survey of 33 human fecal samples from the patient's vicinity all tested negative,while examination of 10 samples of water buffalo feces from around the residence found Fasciola eggs in eight samples(80％),confirmed as Fasciola gigantica through ITS2 gene amplification and sequencing;microscopic examination of 200 specimens of Galba pervia and 20 physaacuta revealed no positive findings;Additionally,observation of 10 samples of pickled Colocasia gigantea(a local edible plant)that the patient had cultivated and consumed raw revealed no Fasciola metacercariae attachment.The results indicate that this case was an accidental case of Fasciola infection.Due to the lack of specificity in clinical symptoms,it is necessary to combine stool examination for Fasciola eggs and molecular diagnosis to confirm the condition.
NETL
NSTL
万方数据
