Differential expression analysis of IL-8 and STAT3 in active tuberculosis patients and its value in adjuvant diagnosis
Objective To compare the differences in the expression of interleukin-8(IL-8)and signal transducer and activator of transcription 3(STAT3)among three groups of individuals:patients with multi-drug resistant tuberculosis(MDR-TB),patients with drug-susceptible tuberculosis(DSP-TB),and healthy controls.By analyzing the differences between these groups,the study seeks to provide a reference basis for clinical auxiliary diagnosis of new type tuberculosis.Methods The differences in mRNA expression profiles of chip from peripheral blood mononuclear cells(PBMCs)among three groups were analyzed.MDR-TB and DSP-TB patients were selected as study subjects,with healthy individuals serving as a control group,and the serum IL-8 concentration of each group was measured using an ELISA assay to compare differences between groups.Additionally,PBMCs from all groups were isolated and total RNA was extracted for analysis of IL-8 and STAT3 mRNA expression using quantitative real-time PCR(Q-PCR).Differences in STAT3 protein expression among the groups were also compared by Western blot assay.Results The serum IL-8 concentration in the MDR-TB and DSP-TB groups was significantly higher than that in the healthy control group,with a statistically significant difference(P＜0.05).The relative expression level of IL-8 mRNA in PBMCs of the MDR-TB group and DSP-TB group was also significantly higher than that of the healthy control group,with a statistical significance(P＜0.05).Both Q-PCR and Western Blot results revealed that the expression of STAT3 in the healthy,DSP-TB and MDR-TB groups sequentially increased,presenting statistically significant differences between the groups(P＜0.05),consistent with the mRNA chip results.Conclusions The expression of IL-8 in active tuberculosis patients(ATB)is significantly increased,which can be used as a marker to distinguish ATB from healthy populations.The changes of STAT3 concentration not only serve as a reference for the diagnosis of ATB but can also distinguish between DSP-TB and MDR-TB populations.The combined detection of IL-8 and STAT3 holds promise as a clinical auxiliary diagnostic basis for different tuberculosis patients.
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