目的 了解武夷山自然保护区蚊虫种类分布特征和重要蚊种的种群遗传多样性,为该地区蚊媒传染病防制提供科学依据.方法 2022-2023年在武夷山自然保护区内选择16个采样点,幼虫采集使用吸管法、成蚊采集使用诱蚊灯法.利用形态特征和基于细胞色素氧化酶亚基Ⅰ(cytochrome c oxidase subunitⅠ,COⅠ)和细胞色素氧化酶亚基Ⅱ(cytochrome c oxidase subunit Ⅱ,COⅡ)的分子鉴定技术相结合鉴定蚊种.利用ClustalX、DnaSP 5.0、Network 4.6.1.0等软件进行日本伊蚊(Ae.japonicus)、白纹伊蚊(Ae.albopoctus)、骚扰阿蚊(Ar.subalbatus)种群遗传分化程度和基因流的分析.结果 经鉴定,本研究在武夷山自然保护区采集的蚊虫隶属于蚊科8属30种.其中伊蚊属10种,库蚊属13种,阿蚊属1种,直角蚊属1种,蓝带蚊属1种,杵蚊亚属1种,按蚊属1种,巨蚊属2种.3种重要蚊虫种群遗传结构分析结果表明,日本伊蚊单倍体多样性指数为0.994 7,种群间普遍达到中等分化程度,种群6与种群12之间遗传分化程度较高;白纹伊蚊单倍体多样性指数为0.634 0,种群1和2与各种群之间遗传分化程度较大;骚扰阿蚊单倍体多样性指数为0.703 3,种群2与种群3、种群4、种群6、种群7之间遗传分化程度较大,基因流较小.结论 武夷山自然保护区蚊虫物种丰富.本研究对蚊种组成、分布开展了较为全面的调查,分析了日本伊蚊、白纹伊蚊、骚扰阿蚊重要蚊种的种群遗传结构特征,为当地蚊媒生态研究和蚊媒疾病防控等方面提供了科学数据参考.
Survey of mosquito species and analysis of genetic structure of important mosquito species populations in Wuyi Mountain Nature Reserve
Objective To elucidate the distribution characteristics of mosquito species and the population genetic diversity of important mosquito species in the Wuyi Mountain Nature Reserve,with the aim of providing a scientific basis for the prevention and control of mosquito-borne diseases in the region.Methods Within Wuyi Mountain Nature Reserve 2022-2023,16 sampling sites were selected,where larvae were collected using the pipette method and adult mosquitoes were collected using the mosquito trap lamp method.Mosquito species identification was accomplished by integrating morphological characteristics and molecular identification techniques based on COⅠand COⅡ gene sequences.Software tools such as ClustalX,DnaSP v5.0,and Network v4.6.1.0 were utilized to analyze the genetic differentiation(Fst),gene flow(Nm)among populations of Aedes japonicus,Aedes albopictus and Armigeres subalbatus.Results The mosquito specimens collected from the Wuyi Mountain Nature Reserve were identified as 30 species belonging to 8 genera within the Culicidae family.Among them,there were 10 species of Aedes,13 species of Culex,1 species each of Armigeres,Orthopodomyia,Uranotaenia,Tripyeroides,and Anopheles,and 2 species of Toxorhynchites.Analysis of the genetic structure of important mosquito populations showed that the haplotype diversity index(Hd)of Ae.japonicus was 0.994 7,with a generally moderate degree of differentiation between populations and a higher degree of genetic differentiation between populations 6 and 12;Aedes albopictus had a haplotype diversity index of 0.634 0,with significant genetic differentiation between populations 1 and 2 compared to other groups;Armigeres subalbatus had a haplotype diversity index of 0.703 3,with substantial genetic differentiation and limited gene flow between population 2 and populations 3,4,6,and 7.Conclusions The mosquito species are rich in Wuyi Mountain Nature Reserve.A comprehensive survey of the composition and distribution of mosquito populations was carried out,and the characteristics of the population genetic structure of the important species of Ae.japonicus,Ae.Albopictus,and Ar.subalbatus populations were analyzed,providing valuable scientific data reference for local mosquito-borne ecological research and disease prevention and control.
NETL
NSTL
万方数据
