Molecular epidemiological characteristics of an outbreak caused by human parainfluenza virus type 1 in Beijing
Objective To conduct an epidemiological investigation and etiological analysis of an outbreak of human parainfluenza virus in Beijing,providing scientific evidence for the prevention and control of the epidemic.Methods A field epidemiological investigation was conducted on fever cases in kindergartens.Throat swab samples were collected from the cases,and real-time fluorescence quantitative polymerase chain reaction was used for pathogen screening.For samples that tested positive for human parainfluenza virus type 1(HPIV1),the HN and F genes were amplified and sequenced,and the genetic characteristics of the virus were analyzed using bioinformatics software.Results All the 12 children affected in this outbreak exhibited symptoms of fever,and additional symptoms included sore throat(7/12),cough(5/12),and runny nose(5/12).Seven children were positive for HPIV1 nucleic acid and negative for other pathogens.The phylogenetic tree showed that the strains in this study belonged to clade 2.3.There were four specific substitution sites(G5E,V22A,P29S,T68A)in the HN gene and three specific substitution sites(N333S,T475K,I509M)in the F gene of the strains,differentiating these strains from others within clade 2.Conclusions This outbreak was caused by HPIV1.The epidemiological investigation and genetic characteristics analysis of HPIV1 could help to trace the source of the outbreak and prevent it.
Human parainfluenza virus type 1fever outbreaksgenetic characteristicsBeijing
万方数据
维普
