Effects of Haihuang Bufei Formula on inflammatory factors and T lymphocyte subsets in silicotic rats
Objective To investigate the effects of Haihuang Bufei Formula on inflammatory factors and T lymphocyte subsets in silicotic rats,and to further explore the therapeutic effects of Haihuang Bufei Formula.Methods The silicotic rat models were constructed by intratracheal instillation of silicon dioxide(SiO2)suspension.After 24 hours of modeling,the rats were randomly divided into normal control group,model group,positive drug control group,and low,medium,high dose group of Haihuang Bufei Formula.Each group contained 10 rats,which were administered once daily for one month.One hour after the last administration,the rats were sacrificed.Lung index,serum tumor necrosis factor α(TNF-α),interleukin 1β(IL-1β),hydroxyproline(HYP),superoxide dismutase(SOD),and malondialdehyde(MDA)contents in lung tissue were measured.Hematoxylin-eosin(HE)and Masson staining methods were used to observe the pathological changes in lung tissues.Flow cytometry was used to detect the T lymphocyte subsets in the peripheral blood of rats.Results Compared with the model group,the lung index,serum IL-1β,lung tissue HYP,and MDA contents in the middle and high dose groups of Haihuang Bufei Formula treatment were significantly reduced(P<0.05).In the high-dose group,the serum TNF-α content decreased to(238.64±51.80)ng/L,and the lung tissue SOD content increased to(55.30±13.77)pg/mL(P<0.05).HE and Masson's staining results indicated that the medium and high doses of Haihuang Bufei Formula could alleviate pathological damage of silicosis and fibrosis in lung tissue.Flow cytometer analysis showed that the percentage of CD8+T cells in the high-dose group of Haihuang Bufei Formula was significantly decreased(P<0.05).The ratio of CD4+/CD8+increased to(1.44±0.19)(P<0.01),and the percentage of CD4+T cells showed no statistically significant change among groups(P>0.05).Conclusions Haihuang Bufei Formula has a protective effect on silicotic rats by reducing collagen content,alleviating pathological damage and fibrosis of lung tissue,which may be related to inhibiting the expression of inflammatory factors,reducing oxidative stress,and regulating the immune system.
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