Epidemiology,molecular characteristics and antibiotic susceptibility analysis of Neisseria meningitidis serogroup Y in Hainan Province
Objective To understand the epidemiology,molecular characteristics,and antibiotic susceptibility analysis of Neisseria meningitidis serogroup Y (NmY) in Hainan Province,and to provide a scientific basis for the prevention,control,and therapy of meningococcal disease caused by NmY. Methods Blood samples from two patients with meningitis discovered in Haikou City in May 2022,as well as swab samples from close contacts,were subjected to Nm isolation and culture. Species identification of the suspected Nm isolates was performed using biochemical tests and conventional polymerase chain reaction. The serogroups and genogroups of Nm isolates were determined by slide agglutination and real-time PCR,respectively. Whole genome sequencing and analysis were performed on seven Nm isolates. The susceptibility to thirteen antibiotics was tested using E-test. Results Both patients were teenagers. A total of seven Nm strains were obtained from the patients and their close contacts,with two strains from the patients and five from the close contacts. The serogrouping results were consistent with the gene grouping results,all identifying the isolates as serogroup Y Nm. The GC content of all NmY isolates ranged from 51.84％to 51.86％,and all isolates belonged to sequence type ST-1655,which is part of the ST-23 clonal complex. The types of outer membrane proteins of all isolates were identical porA (P1.5-1,10-1),porB (3-117),and fetA (F4-1). The sequences of antibiotic-related genes of all isolates were identical. The isolates were all susceptible to penicillin,ampicillin,cefotaxime,ceftriaxone,meropenem,chloramphenicol,azithromycin,minocycline,rifampicin,ciprofloxacin,nalidixic acid,and levofloxacin,but resistant to trimethoprim-sulfamethoxazole. Conclusions Cases of infection with ST-1655 serogroup Y N. meningitides have been identified in Hainan Province. It is necessary to strengthen the surveillance of meningococcal disease and promote the meningococcal ACWY quadrivalent meningococcal vaccine to prevent meningococcal disease caused by NmY.
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