首页|AQP4 expression and its correlation with the Lac and NAA using proton magnetic resonance spectroscopy after rat cerebral ischemia
AQP4 expression and its correlation with the Lac and NAA using proton magnetic resonance spectroscopy after rat cerebral ischemia
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NETL
NSTL
万方数据
维普
Objective: To determine whether AQP4 expression is associated with lactate (Lac) and Nacetyl aspartate (NAA) and with apparent diffusion coefficient (ADC) abnormality after rat cerebral ischemia. Methods: The time courses of ADC and lactate and NAA assessed by proton magnetic resonance spectroscopy (1HMRS) were investigated at the time point of 6 h, and 1, 3, 7 d after rat cerebral ischemia induced by middle cerebral artery occlusion. Expression of AQP4 mRNA and protein were measured using RT-PCR and Western blot analysis respectively. Results: Significant reductions of NAA concentration and increases of lactate concentration were found after rat cerebral ischemia. The expressions of AQP4 mRNA and protein were increased at 6 h, and reached the peak at 1-3 d, then began to decrease at 7 d after rat cerebral ischemia. The expression of AQP4 was significantly correlated with NAA (rRT =-0.856, rw =-0. 927, P＜0. 01), and with lactate (rW=0. 473, rRT=0. 413, P＜0. 05), and with ADC values during the period of 1-7 d after rat cerebral ischemia (rW=0. 984, rRT= -0. 925, P＜0.05). In addition, correlations between Lac and the ADC values(r=-0. 677, P＜0. 05)and between NAA and ADC values during the period of 1-7 d after rat cerebral ischemia (r= 0. 909, P＜0.05) were also observed. Conclusion: The data suggest that AQP4 is involved in the transport of water when brain edema is formed and cell membrane integrity is lost.
cerebral ischemiabrain edemamiddle cerebral artery occlusionproton magnetic resonance spectroscopyaquaporin
ZHOU Ren-lan、XIE Peng
展开 >
Department of Neurology, First affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China
NETL
NSTL
万方数据
维普
