Abstract
Objective:Assessment of digestion colonic flora lactulose can be induced to produce hydrogen and prove lactulose with a potential anti-inflammatory effects.Methods:We estabfished a mouse model of human ulcerative colitis (UC) by supplying mice with water containing dextran sulfate sodium (DSS).At 7-day,DSS-induced pathogenic outcomes,including loss of body weight,increase of colitis score,pathogenic shortening of colon length,elevated level of TNF-α,IL-1β,malondialdehyde (MDA) and myeloperoxidase (MPO) deficiency in colon lesions,were significantly suppressed by lactulose administration and intraperitoneally injected hydrogen water.Results:After eliminating colon microflora by antibiotics,the anti-inflammatory effects of lactulose were significantly compromised.Histological analysis also revealed that the DSS mediated colonic tissue destruction accompanied by neutrophils infiltration was remarkably suppressed by lactulose and hydrogen water.Conclusion:The present study indicated that lactulose can prevent the development of DSS-induced colitis and alleviate oxidative stress state in colitis.
基金项目
Supported by Creativity and Innovation Training Program of Second Military Medical University(ZD2010009)
NETL
NSTL
维普
万方数据