Abstract
Background:Thermotherapy has already been proved effective for the treatment of various tumors,including glioma.This study was performed to determine whether tumor necrosis factor-alpha was involved in the regulation of this biological process.Methods:RT-PCR and immunocytochemistry were used to investigate the levels of tumor necrosis factor-alpha mRNA and heat shock factor-1 protein,respectively,in glioma cells.Radioimmunoassay was used to dynamically monitor contents of TNF-α in nutrient fluid for C6 cells after hyperthermia treatment.Crystal violet staining method was used to detect glioma invasiveness.Results:The most obvious increase of heat shock factor-1 protein and tumor necrosis factor-alpha mRNA in C6 cells were observed at 30 min and 60 min after hyperthermia,respectively.In addition,the radioactivity of tumor necrosis factor-alpha in C6 cells' culture fluid also reached peak at 120 min ofhyperthermia.The glioma invasiveness decreases and the concentration of tumor necrosis factor-alpha reached the maximum at 120 min ofhyperthermia.Conclusion:Our results showed that the hyperthermia-mediated glioma invasiveness decreases was due to accelerated release of tumor necrosis factor-alpha,which could cause the decreases of glioma invasiveness by promoting the release heat shock factor-1 from neurospongioma cells.
基金项目
Supported by the Scientific Research Foundation of Hebei Provincial Department of Health,and the Project of Science and Technolo(10140201A-15)
NETL
NSTL
维普
万方数据