To analyze the stability ofLactobacillus rhamnosus LV108 in a continuous passaging process.The fermentation properties and probiotic properties of lactic acid bacteria strains were measured,and RAPD method were used to analysis the different algebraic genomic changes in LV108.The results showed that Lactobacillus rhamnosus LV108 have a good stability.pH,acidity,water holding capacity viscosity of the fermented milk were not significantly changed (P＞0.01).LV108 had good acid and bile salt resistant ability,which were cultured 2h in Medium of pH3.0 and medium containing 0.3％ bile salts,the survival rates were greater than 90％ and 45％ respectively.It' s antimicribial activity were not changed significantly (P＞0.01),Escherichia coli and Staphylococcus aureus were more moderate suppressed by LV108.LV108 had stable enzyme activity of ALDC,LDH and α-Gal (P＞0.01).The enzyme activity were respectively at around 0.080～0.087 U/mL,0.331-0.334 U/mL and 1.326-1.341 U/mL.20 random primers were used to amplify genomic DNA,and all kinds of amplified bands in fingerprint were analyzed.Similarity of genome were between 0.9840-0.9973,and each generation strains genome have great similarity during subculture.Therefore,as a good fermentation strain,LV108 could be used in industrial production.
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维普
万方数据
