乳铁蛋白改善高脂饮食大鼠肝脏胆固醇积累的作用研究
Effect of lactoferrin on high-fat diet induced hepatic cholesterol accumulation in rats
吴昀宣 1李德明 1何宇然 1徐加英 2童星 3秦立强1
作者信息
- 1. 苏州大学公共卫生学院,江苏苏州 215123
- 2. 苏州大学放射医学与防护学院,江苏苏州 215123
- 3. 苏州大学苏州医学院实验中心,江苏苏州 215123
- 折叠
摘要
研究了乳铁蛋白(Lf)对高脂饮食诱导肥胖大鼠肝脏胆固醇代谢的影响.30只SD大鼠随机分为对照组(CON)、高脂饮食组(HFD)和乳铁蛋白干预组(HFD+Lf),HFD+Lf组的高脂饲料中0.4％酪蛋白替换成Lf.干预16周后,苏木精-伊红染色观察大鼠肝脏脂肪累积,PCR法测定肝脏和小肠组织中胆固醇外流相关基因肝X受体α(LXRα)、ATP结合盒转运蛋白(ABCA1、ABCG1和ABCG5)、肝脏胆固醇合成相关基因甾醇调节元件结合转录因子2CSREBP2)以及肠道胆固醇吸收相关蛋白尼曼-匹克C1型类似蛋白1(NPC1L1)的mRNA水平.结果显示Lf干预缓解了 HFD诱导的肝脏脂肪积累,上调了肝脏和肠道的LXRα、ABCA1和ABCG5的mRNA水平,下调了肝脏SREBP2及肠道NPC1L1的mRNA表达水平.因此,Lf可能通过增加胆固醇外流,减少肝脏胆固醇合成和小肠胆固醇吸收,从而改善高脂饮食引起的肝脏胆固醇积累.
Abstract
The effect of lactoferrin(Lf)on hepatic cholesterol accumulation in high-fat diet-induced obese rats was studied.Thirty SD rats were randomly divided into three groups,namely the control group(CON),the high-fat diet group(HFD)and the lactoferrin group(HFD+Lf).For HFD+Lf group,0.4％casein in the diets was replaced by Lf.After 16 weeks,hepatic fat accumulation was observed by HE staining.PCR method was used to determined the mRNA levels of liver X receptor α(LXRα),and ATP-binding cassette transporter proteins(ABCA1,ABCG1 and ABCG5)which are involved in intestinal cholesterol efflux in the liver and small intestine,sterol regulatory element binding transcription factor 2(SREBP2)which is involved in cholesterol synthesis in liver,and Niemann-Pick C1-like protein(NPC1L1)which are involved in intestinal cholesterol absorption in small intestine.AS a result,HFD resulted in the disfunction of hepatic cholesterol metabolism.Lf intervention alleviated the hepatic fat accumulation,significantly upregulated liver and intestinal LXRα,ABCA1 and ABCG5 gene expression,downregulated hepatic SREBP2 and intestinal NPC1L1 gene expression.Thus,Lf improves the disruption of cholesterol metabolism caused by a HFD via increasing cholesterol efflux,and reducing hepatic cholesterol synthesis and intestinal cholesterol absorption.
关键词
乳铁蛋白/胆固醇/肝脏/小肠Key words
lactoferrin/cholesterol/liver,small intestine引用本文复制引用
基金项目
国家自然科学基金(82173502)
国家自然科学基金(82003438)
出版年
2023
NETL
NSTL
万方数据