基于多孔金@铂纳米酶的比色型生物传感器用于检测牛乳中鼠伤寒沙门氏菌研究
Colorimetric biosensor based on porous gold@platinum nanocatalysts for detection of Salmonella typhimurium in milk
苑懿 1黄羽文 1邢巾 1张宇1
作者信息
- 1. 东北农业大学食品学院 乳品科学教育部重点实验室,哈尔滨 150030
- 折叠
摘要
建立了 一种快速、灵敏检测牛乳中鼠伤寒沙门氏菌ATCC 14028(Salmonella typhimurium,S.typhimurium)的方法.利用鼠伤寒沙门氏菌多克隆抗体(Anti-S.typhimurium-pAb)修饰磁珠(Magnetic nanoparticles,MNPs)形成免疫磁珠(MNPs@pAb),鼠伤寒沙门氏菌单克隆抗体(Anti-S.typhimurium-mAb)标记多孔金@粕纳米酶(gold@platinum nanocatalysts,Au@PtNCs)形成具有催化性能的免疫探针Au@PtNCs@mAb,与鼠伤寒沙门氏菌最终反应形成MNPs@pAb-S.typhimurium-Au@PtNCs@mAb复合物后磁分离富集,在过氧化氢存在下可催化显色底物3,3',5,5'-四甲基联苯胺(H2O2-TMB)生成蓝色物质,并在652 nm处产生较强的特征吸收峰.在最佳条件下,最低检测限为81 CFU/mL,线性范围为97～9.7×105 CFU/mL,实际牛乳样本加标实验中加标回收率为96.75％～101.75％,变异系数(CV)为1.10％～5.13％.该方法建立的比色型生物传感器具有简单、快速、灵敏等特性,可用于检测乳品中的鼠伤寒沙门氏菌.
Abstract
A rapid and sensitive method for the detection of Salmonella typhimurium(S.typhimurium)ATCC 14028 in milk was established.Using SS.typhimurium polyclonal antibody(Anti-S.typhimurium pAb)to modify magnetic nanoparticles(MNPs)to form immune magnetic beads(MNPs@pAb),and using S.typhimurium monoclonal antibody(Anti-S.typhimurium-mAb)to label porous gold@platinum nanocatalysts(Au@Pt-NCs)to form immune probes Au@PtNCs@mAb with catalytic properties,which reacted withS,typhimurium to form MNPs@pAb-S.ty-phimurium-Au@PtNCs@mAb complexes and undergo magnetic separation and enrichment.In the presence of hydrogen peroxide,the chro-mogenic substrates 3,3',5,5'tetramethylbenzidine(H2O2-TMB)can be catalyzed to generate blue substances,and a strong characteristic absorption peak is generated at 652 nm.Under optimal conditions,the minimum detection limit is 81 CFU/mL,and the linear range is 97-9.7×10bCFU/mL,the recovery rate of standard addition in the actual milk sample standard addition experiment is 96.75％-101.75％,and the coefficient of variation(CV)is 1.10％-5.13％.The colorimetric biosensor established by this method is simple,rapid and sensitive.It can be used to detectS,typhimurium in dairy products and has reference significance for food safety detection.
关键词
多孔金@铂纳米酶/鼠伤寒沙门氏菌/比色/快速检测Key words
porous gold@platinum nanocatalysts/S.typhimurium,colorimetry/rapid detection引用本文复制引用
出版年
2023
NETL
NSTL
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