In this study,chemically defined medium capable of promoting the growth of Streptococcus thermophilus grx02 and its variable temperature culture method were studied.The composition of chemical defined medium was determined by a single deduction experiment.The chemically defined medium component concentration and variable temperature culture methods were further optimized to increase the number of viable bac-teria of S.thermophilus grx02.The results showed that SS.thermophilus grx02 chemically defined proliferation medium is composed of 1 carbohy-drate,16 amino acids,7 vitamins,3 minerals,4 inorganic salts,2 organic salts and deionized water;The initial temperature,terminal phase and loga-rithmic terminal phase temperatures were 39,37 ℃ and 39 ℃ of the culture conditions.Compared with that before optimization,the biomass of SS.thermophilus grx02 increased by 1.63 times reaching 1.40,and the number of viable bacteria was 9.04 log CFU/mL.This study can provide some reference for the production and metabolism and function of Streptococcus thermophilus
关键词
嗜热链球菌/化学限定培养基/变温培养
Key words
Streptococcus thermophilus/chemically defined medium/temperature-shift cultrue
维普
万方数据