Study on chemically restricted proliferation medium and allothermic culture strategies in Streptococcus thermophilus grx02
In this study,chemically defined medium capable of promoting the growth of Streptococcus thermophilus grx02 and its variable temperature culture method were studied.The composition of chemical defined medium was determined by a single deduction experiment.The chemically defined medium component concentration and variable temperature culture methods were further optimized to increase the number of viable bac-teria of S.thermophilus grx02.The results showed that SS.thermophilus grx02 chemically defined proliferation medium is composed of 1 carbohy-drate,16 amino acids,7 vitamins,3 minerals,4 inorganic salts,2 organic salts and deionized water;The initial temperature,terminal phase and loga-rithmic terminal phase temperatures were 39,37 ℃ and 39 ℃ of the culture conditions.Compared with that before optimization,the biomass of SS.thermophilus grx02 increased by 1.63 times reaching 1.40,and the number of viable bacteria was 9.04 log CFU/mL.This study can provide some reference for the production and metabolism and function of Streptococcus thermophilus
Streptococcus thermophiluschemically defined mediumtemperature-shift cultrue
万方数据
维普
