In this study,using mammary epithelial cells of dairy cows as a experimental model to investigate the regulation of prolactin on RANKL gene promoter activity.The expression of RANKL protein induced by prolactin was detected by Western Blot.The RANKL promoter luciferase vectors were constructed,and the the effect of prolactin on RANKL promoter activity was detected by dual luciferase assay.The mutant RANKL promoter luciferase vector was constructed by site-directed mutation synthesis,and the effect of prolactin on the activity of mutant RANKL pro-moter was detected by dual luciferase assay.The results showed that prolactin significantly increased the promoter activity and protein expression of RANKL in mammary epithelial cells.The prolactin response element was located in the-826～-814 bp region of dairy cows RANKL gene pro-moter.
关键词
奶牛乳腺上皮细胞/催乳素/RANKL/STAT5a
Key words
mammary epithelial cells of dairy cows/prolactin/RANKL/STAT5a
维普
万方数据