Screening and qualitative analysis of common antibiotic resistance encoding genes in commercial sterilized milk
To explore the distribution and prevalence characteristics of tetracyclines,aminoglycosides,and β-lactam antibiotics resistance genes(ARGs)in common commercial sterilized milk.Taking 12 kinds ofsterilized milk that commonly sold on the market as samples,the microbial DNA in the milk was extracted and purified by CTAB method,the purity and concentration of DNA were measured by an ultramicro spectrophotometer for PCR amplification.After PCR products of genes were sequenced and submitted to GenBank for sequence alignment,the distribution and prevalence characteristics of ARGs were analyzed,the correlation analysis was made on the ARGs detection rate,milk brand,packaging and product type of milk samples using SPSS software.The results showed that a variety of ARGs was detected from common commercial sterilized milk,the top five ARGs were strB(93.75％),aadB(92.71％),sul1(85.42％),blaTEM(82.29％)and tetC(58.33％),respectively.Among 35 screened ARGs,only 5 genes including blaVEB,blaPER,blaDHA,blaCMY2 and blaGIM were not detected.The detection rates ofaadA,aadB,strA,strB and other ARGs were found to be significantly correlated with the milk brand,packaging type,milk original place and shelflife of the commercial sterilized milk.There are many kinds of ARGs in common commercial sterilized milk,and ARGs are different in different kinds of milk indicating that the residual ARGs in sterilized milk is a problem that cannot be ignored,and it is worthy to further study from the perspective of food safety.
万方数据
维普
