模拟肠液培养基中壳寡糖对益生菌生长的影响
Effects of Chito-oligosaccharides on the growth of probiotics in simulated intestinal medium
张聪春 1康文丽 2韩一凤 3马文龙 1李意思 2潘丽娜 2汪家琦 2顾瑞霞1
作者信息
- 1. 扬州大学食品科学与工程学院 江苏乳品生物技术与安全控制重点实验室,江苏扬州 225127
- 2. 澳优乳业(中国)有限公司 澳优食品与营养研究院,长沙 410203
- 3. 江苏旅游职业学院,江苏扬州 225000
- 折叠
摘要
文章探究了模拟肠液培养基中壳寡糖对益生菌生长的影响,为益生菌、壳寡糖合生元的开发提供理论依据.将益生菌接种至含不同浓度壳寡糖的模拟肠液培养基中培养,通过测定菌体密度(△OD600)、活菌数的变化,测定△OD260的变化以及用扫描电子显微镜表征细胞膜的完整性,明确模拟肠液培养基中壳寡糖对益生菌生长、细胞膜完整性的影响.结果显示8株专利益生菌都可以在模拟肠液培养基中正常生长,都不能以壳寡糖为唯一碳源生长.自然培养过程模拟肠液培养基中壳寡糖抑制8株专利益生菌的生长;壳寡糖处理导致益生菌细胞膜表面塌陷及褶皱,破坏了益生菌细胞膜的完整性.偏碱性模拟肠液培养基中壳寡糖对8株专利益生菌的生长没有统一的抑制作用.偏碱性模拟肠液环境下,壳寡糖对益生菌生长的抑制作用较弱,或没有抑制作用,可以将益生乳酸菌与壳寡糖复配开发合生元,用于调节肠道微生态.
Abstract
The objectice of this paper is to explore the effects of Chito-oligosaccharide on the growth of probiotics in stimulated intestinal fluid culture medium,so as to provide a theoretical basis for the development of probiotic and Chito-oligosaccharide synbiotics.Method:Eight probi-otics were inoculated into simulated intestinal fluid culture medium containing different concentrations of Chito-oligosaccharide for cultivation.The changes of cell density(△OD600),viable bacteria count,and the absorbance of culture at △OD260 nm were measured,as well as the integrity of cell membrane was characterized by scanning electron microscope.Results:all 8 probiotics grew normally in simulated intestinal fluid culture medium,and cannot grow with Chito-oligosaccharide as the sole carbon source.Chito-oligosaccharide inhibited the growth of 8 probiotics in simulation simulated intestinal fluid culture medium under natural cultivation process.Chito-oligosaccharide treatment led to surface collapse and wrinkles of probiotic cell membranes,disrupting the integrity of probiotic cell membranes.However,there was no unified inhibitory effect of Chi-to-oligosaccharide on the growth of 8 patented probiotics in alkaline simulated intestinal fluid culture medium.Conclusion:under alkaline simulat-ed intestinal fluid environment,Chito-oligosaccharide has a weak or no inhibitory effect on the proliferation of probiotics.Probiotics can be com-bined with Chito-oligosaccharide to develop synbiotics for regulating intestinal microecology.
关键词
壳寡糖/益生菌/抑菌/细胞膜/模拟肠液培养基Key words
Chito-oligosaccharide/probiotics/bacteriostasis/cell membrane/simulated intestinal juice引用本文复制引用
基金项目
扬州市-扬州大学合作共建科技创新平台(YZ2020265)
湖南省营养健康品工程技术研究中心创新平台与人才计划(2019TP2066)
江苏省高等学校自然科学研究项目(21KJB550009)
扬州大学大学生科技创新基金项目(X20220938)
出版年
2024
NETL
NSTL
维普
万方数据