Study on characteristics and differences of microbial composition in milk of healthy cows and mastitic cows
The occurrence of cow mastitis is related to a variety of pathogenic microorganisms.Analysizing the characteristics of milk flora is of great significance for establishing mastitis prevention and control system,as well as improving safety and quality of dairy products.This study analyzed the structural diversity and richness of microbial communities in bovine milk from 17 healthy cows and 10 mastitic cows in Henan Province by 16S rRNA sequencing with V3-V4 gene region.The results showed that there were significant differences in the relative abundance of microbial communities at the levels of 11 phyla,24 classes,37 orders,82 families,and 184 genera.The diversity and richness of microbial flora structure in the mastitis milk were significantly lower than that of healthy cows.Firmicutes,Proteobacteria and Actinobacteria are the phyla with higher relative abundance in milk of mastitis cows.After mastitis,the abundance of Proteobacteria and Tenericutes decreased significantly,the abundance of Synergistetes significantly increased.The relative abundance of Streptococcaceae and Streptococcus in the milk of mastitis cows increased,while the relative abundance of Corynebacteriaceae,Ralstonia and Pseudomonas decreased.There were four Psychrophilic bacteria in the dominant genera.The total abundance of Psychrophilic bacteria in the milk of healthy cows and mastitis cows was 19.5％and 42.0％,respectively.This study provides a theoretical basis for preventing and controlling mastitis in dairy farms,as well as controlling the quality of raw milk.
NETL
NSTL
万方数据
维普
