Establishment of high throughput screening method for γ-aminobutyric acid-producing lactobacillus
Firstly,11 strains of Levilactobacillus brevis were found to produce a large amount of gas,which was enough to push the cover of culture tubes open.Among them,three strains showed a higher pH after 48 h fermentation.Then,thin layer chromatography analysis further proved that only the strains with gas production and higher pH of the fermentation broth could produce gamma-aminobutyric acid(GABA).Mass spectrom-etry showed that the yield of GABA of three L.brevis strains was 124.14,203.00 mg/L and 211.50 mg/L,respectively.Interestingly,high-through-put sequencing analysis found that the gene structure and nucleic acid sequence responsible for GABA synthesis of 11 L.brevis strains were almost same,so it may not be achieved for high-throughput screening GABA-producing strains at the gene level.Based on carbon dioxide production and hydrogen ions consumption during fermentation,a high-throughput method for screening GABA-producing lactobacillus strains was estab-lished.Furthermore,the established method was applied to select GABA-producing Lactiplantibacillus plantarum among 220 bacterial strains,which proved its feasibility.In conclusion,this study reported an available method for quickly screening GABA-producing strains from a large number of lactobacillus with low cost and high throughput characteristics.
NETL
NSTL
万方数据
维普
