The purpose of this experiment was to explore the effect of ellagic acid supplementation on blood biochemical indexes of Ili horses during lactation under grazing conditions,and to provide a research basis for ellagic acid as a feed additive to regulate the health of domestic animals during lac-tation.Eighteen lactating Ili mares were randomly divided into three groups,six in each group,which were respectively divided into control group,test group Ⅰ and test group Ⅱ.Under the same feeding and management conditions,the control group mares were not treated with any additives.Each mare in test group Ⅰ was fed with ellagic acid 15 mg/d(kg·BW),and each mare in test group Ⅱ was fed with ellagic acid 30 mg/d(kg·BW).The test peri-od is 90 days,and the horse blood samples are collected regularly to determine the relevant indicators.The results showed that the blood glucose and direct bilirubin levels of mares in test group Ⅰ were significantly lower than those in control group(P<0.01);The blood triglyceride level in Test GroupⅡ was significantly higher than that in T est Group Ⅰ(P<0.01).The levels of aspartate aminotransferase and creatine kinase in the blood of mares in test groupⅡ were significantly lowerthan those in the control group(P<0.0 5);The serum alkaline phosphatase of maresin test group Ⅱ was significantly lowerthan that i n control group and test group Ⅰ(P<0.01);Test Group Ⅰ The level of γ-glutamyltransferase was significantly higher than that ofcontrol group and test groupⅡ(P<0.01);the blood lactate dehydrogenase level of mares in test group Ⅱ was significantly lower than that in control group and test group Ⅰ(P<0.01).The blood iron content of mares in test group Ⅰ was significantly higher than that in control group(P<0.01).Therefore,under the conditions of this experiment,supplementing Ili horses with 15 mg/d(kg·BW)ellagic acid during lactation can significantly reduce blood sugar,triglycerides,and direct bilirubin levels,and improve γ-glutamyltransferase and iron content;Supplementing 30 mg/d(kg·BW)ellagic acid can reduce the activities of aspar-tate aminotransferase,alkaline phosphatase,creatine kinase,and lactate dehydrogenase in the blood of mares.