Development of colloidal gold immunochromatographic reagent cards for detection of human lactoferrin
A rapid and quantitative method for the determination of human lactoferrin was developed.Based on colloidal gold immunochromatog-raphy and double antibody sandwich method,a reagent card for the detection of lactoferrin in human milk was developed by optimizing the label-ing and coating conditions,and the performance of prepared reagent card was evaluated.The performance evaluation results of reagent card indicat-ed that the blank limit can be as low as 0.014 mg/mL,the correlation coefficient for sample detection of human milk compared to high-perfor-mance liquid chromatography was 0.92,and there was no cross reaction with bovine lactoferrin,casein andβ-lactoglobulin.This study provides a rapid,simple,and easy to operate method for the detection of human lactoferrin.
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