This article utilized Illumina Miseq high-throughput sequencing technology to conduct an in-depth analysis of microbial diversity in processed milk powder.The research revealed the presence of 21 bacterial phyla and 339 bacterial genera throughout the entire processing.Addi-tionally,we identified four dominant phyla,namely Firmicutes,Proteobacteria,Bacteroidota,and Actinobacteriota,along with dominant genera including Streptococcus,Lactococcus,Enterococcus,and Bacillus.We also discovered the widespread presence of Clade_Ia and Ralstonia genera across all sample groups.Principal component analysis revealed high similarity and stability in microbial compositions across each sample.The species abun-dance heatmap further revealed that raw milk serves as the primary source of microbes,while ingredient processing is a critical step in microbial contamination.The study also indicated a decreasing trend in microbial community structure and diversity during milk powder processing.By ex-ploring the dynamics of microbial changes during processing and the interrelationships between samples,this research lays the foundation for future studies on microbial diversity in milk powder processing.
维普
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