Effect of different methods to the content of pantothenic acid in milk powder based on analysis of variance
The national standard method,microbiological reagent kit method,and water bath extraction national standard method were used to de-termine the pantothenic acid content of quality control samples and milk powder samples,respectively.The results were analyzed and evaluated us-ing SPSS statistical analysis method.The results showed that the correlation coefficient of the standard curve of the national standard method was R2=0.9982,and the correlation coefficient of the reagent kit method was R2=0.9988,indicating high sensitivity of both methods;The RSD of the three methods ranges from 2.10%to 3.11%,all of which are less than 5%;The results of quality control samples and milk powder samples were an-alyzed using SPSS two factor complete random analysis of variance and two factor repeated measurement analysis.It was found that the three dif-ferent methods had no effect on the determination of pantothenic acid content(P>0.05).The personnel compared the experimental data had high precision and accuracy,consistent personnel operation level.The data showed that the laboratory test error was small,and it met the requirements of the method.The results show that there is no significant difference among the three detection methods,based on that,the national standard method was optimized and the efficiency of pretreatment was improved.The best solution can be selected based on actual needs.