Effect of RANKL on LPS-induced inflammatory response of cow mammary epithelial cells
In this study,an inflammation model of cow mammary epithelial cells was established by LPS induction.After RANKL was overex-pressed,MTT assay was used to detect the activity of cow mammary epithelial cells in inflammatory models.And the mRNA expressions of in-flammatory cytokines IL1β,IL6,iNOS and TNF-α in cow mammary epithelial cells were detected by fluorescence quantitative PCR.Further,the effect of RANKL on LPS-induced inflammatory response of cow mammary epithelial cells was investigated.The results showed that RANKL significantly increased the activity of mammary epithelial cells in the mammary epithelial cell inflammation model and significantly decreased the inflammatory cytokines IL1β,IL6,iNOS in the mammary epithelial cell inflammation model after the mammary epithelial cells were treated with 10 μg/mL LPS for 24 h.In addition,the mRNA and protein expression of TNF-α in mammary epithelial cell inflammation models of were sig-nificantly decreased.
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维普
