Effect of infant sheep formula milk powder on antibiotic induced diarrhea in mice
To investigate the effect of infant sheep formula milk powder on antibiotic-induced diarrhea in mice,and to provide experimental basis for the promotion and application of infant sheep formula milk powder.50 KM mice were randomly divided into blank control group,model con-trol group,sheep milk powder low dose group,high dose group and infant formula milk powder group according to gender weight,10 mice in each group,half male and half female.The milk powder group was given 20 mL/kg BW gavage,and the blank and model control groups were given gavage with pure water twice a day for 14 consecutive days.After 7 days of sample administration,the blank control group was given gavage of normal saline,and the other groups were given gavage of cefradine(1.00 g/kg BW)+gentamicin(0.16 g/kg BW),0.4 mL/only,twice a day for 4 consecutive days.After 2 h of antibiotics,the samples were continued to be given.It was used for the determination of fecal water content and in-testinal microbial analysis.The next day after the last sample was given,the levels of serum TNF-α,IL-2,IL-6 and IL-1β were measured by or-bital blood collection.The jejunum and colon tissues were taken for histopathological examination.Result showed that compared with blank con-trol group,fecal water content in model control group was significantly increased(P＜0.05),serum IL-2 level was significantly decreased(P＜0.01),IL-6 and IL-1β levels were significantly increased(P＜0.01),the number of crypts in jejunum and ileum tissue was decreased,intestinal villi was shorter,lamina propria inflammatory cell infiltration and other pathological changes were observed.The structure of intestinal flora changed and the diversity decreased.Compared with model control group,fecal water content in sheep milk powder high-dose group and infant formula milk powder group was significantly decreased(P＜0.05 or P＜0.01),serum IL-2 level was significantly increased(P＜0.05),and IL-6 level was signifi-cantly decreased(P＜0.05).Sheep milk powder can obviously repair jejunum and colonic tissue lesions in mice,and regulate the diversity and composition of intestinal flora caused by antibiotics.Infant sheep formula milk powder may improve intestinal pathological injury of antibiotic-in-duced diarrhea mice by improving intestinal immune function and regulating intestinal flora composition.
infant formula sheep milk powderintestinal floraantibioticsdiarrheamouse
万方数据
维普
