Restorative effect of ampicillin-induced intestinal flora disorders by Lactobacillus paracasei Glory LP16 in BALB/c mice
The aim of this study was to evaluate the ameliorative effect of different concentrations of Lactobacillus paracasei on intestinal dysbiosis us-ing an animal model and to investigate the dose effect.Sixty BALB/c mice were divided into five groups,and the model of intestinal flora disorders was constructed by gavage of ampicillin,while the concentration of Lactobacillus paracasei Glory LP16 was used as the basis of differentiation,and the mice were divided into the control group,the model group,the low-dose group(1.6×106 CFU/only),the middle-dose group(1.6×107 CFU/only),and the high-dose group(1.6×108 CFU/only),body weight changes were measured,and colon tissue and faecal samples were collected for length measure-ments,histopathological analyses,intestinal barrier,intestinal permeability,intestinal microbiota analysis,and short-chain fatty acid determinations.Compared with the model group,the Lactobacillus paracasei Glory LP16-fed group significantly increased the relative abundance of beneficial bacte-ria such as Bifidobacterium and Lactobacillus,restored the colonic tissue structure,increased the expression of intestinal barrier-related genes,increased the content of short-chain fatty acids and maintained the intestinal channel permeability well enough to preserve the intestinal integrity under the medium-dose condition,restored the ampicillin-induced BALB/c mice with disturbed intestinal flora.
万方数据
维普
