超高效液相色谱串联质谱法快速检测液态乳中41种磺胺及喹诺酮类化合物
Rapid determination of 41 sulfonamides and quinolones antibiotics in liquid milk by QuEChERS purification and liquid chromatography tandem mass spectrometry with Scheduled MRM mode
黄珊 1欧阳小艳 1范云燕1
作者信息
- 1. 南宁市疾病预防控制中心,南宁 530023
- 折叠
摘要
文章建立了使用C18粉末分散固相萃取净化,超高效液相色谱串联三重四级杆质谱仪快速检测液态乳中41种磺胺及喹诺酮类化合物的方法.样品用2％的甲酸乙腈提取,离心后取上清液用C18粉末净化,净化后的样品稀释一倍后用超高效液相色串联三重四级杆质谱仪在Scheduled MRM模式下检测,以基质匹配的标准曲线定量.结果表明,41种磺胺及喹诺酮化合物在检测范围内线性关系良好,各化合物标准曲线相关系数r≥0.998,样品加标回收率为78％～116％,RSD为0.5％～9.9％,检出限范围0.05～0.20 μg/kg,定量限范围0.15～0.60 μg/kg.该方法快速、简单、灵敏度高、稳定性好、检测成本低,适用于液态乳中41种磺胺及喹诺酮类化合物快速检测.
Abstract
In this study,a method was developed for the rapid detection of 41 sulfonamides and quinolones in liquid milk by ultra-performance liquid chromatography tandem triple quadrupole-mass spectrometry(UPLC-MS/MS)using C18 powder purification.Sulfonamidesand and quinolones were extracted from milk with 2％formic acid acetonitrile,followed by centrifuging,purifying and diluting,at last,the sample was anal-ysed with UPLC-MS/MS on Scheduled MRM mode,quantitative by matrix matching standard curve.The results showed that 41 sulfonamides and quinolones had good linearity in the detection range,and the correlation coefficient of each compound r≥0.998.The average recovery of the method was in the range of 78％-116％,with the relative standard deviation of 0.5％-9.9％.The limits of detection range were 0.05-0.20 μg/kg,limits of quantification range were 0.15-0.60 μg/kg.The method had the characteristics of easy operation,fast speed,high accuracy,good repeata-bility,high sensitivity and low cost.It is suitable for the rapid detection of 41 sulfonamides and quinolones in liquid milk.
关键词
超高效液相色谱串联三重四级杆质谱仪/C18/快速检测/磺胺/喹诺酮Key words
ultra-high performance liquid chromatography tandem triple quadrupole mass spectrometer/C18/rapid detection/sulfonamides/quinolones引用本文复制引用
出版年
2024
NETL
NSTL
万方数据