首页|超高效液相色谱串联质谱法快速检测液态乳中41种磺胺及喹诺酮类化合物

超高效液相色谱串联质谱法快速检测液态乳中41种磺胺及喹诺酮类化合物

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文章建立了使用C18粉末分散固相萃取净化,超高效液相色谱串联三重四级杆质谱仪快速检测液态乳中41种磺胺及喹诺酮类化合物的方法.样品用2%的甲酸乙腈提取,离心后取上清液用C18粉末净化,净化后的样品稀释一倍后用超高效液相色串联三重四级杆质谱仪在Scheduled MRM模式下检测,以基质匹配的标准曲线定量.结果表明,41种磺胺及喹诺酮化合物在检测范围内线性关系良好,各化合物标准曲线相关系数r≥0.998,样品加标回收率为78%~116%,RSD为0.5%~9.9%,检出限范围0.05~0.20 μg/kg,定量限范围0.15~0.60 μg/kg.该方法快速、简单、灵敏度高、稳定性好、检测成本低,适用于液态乳中41种磺胺及喹诺酮类化合物快速检测.
Rapid determination of 41 sulfonamides and quinolones antibiotics in liquid milk by QuEChERS purification and liquid chromatography tandem mass spectrometry with Scheduled MRM mode
In this study,a method was developed for the rapid detection of 41 sulfonamides and quinolones in liquid milk by ultra-performance liquid chromatography tandem triple quadrupole-mass spectrometry(UPLC-MS/MS)using C18 powder purification.Sulfonamidesand and quinolones were extracted from milk with 2%formic acid acetonitrile,followed by centrifuging,purifying and diluting,at last,the sample was anal-ysed with UPLC-MS/MS on Scheduled MRM mode,quantitative by matrix matching standard curve.The results showed that 41 sulfonamides and quinolones had good linearity in the detection range,and the correlation coefficient of each compound r≥0.998.The average recovery of the method was in the range of 78%-116%,with the relative standard deviation of 0.5%-9.9%.The limits of detection range were 0.05-0.20 μg/kg,limits of quantification range were 0.15-0.60 μg/kg.The method had the characteristics of easy operation,fast speed,high accuracy,good repeata-bility,high sensitivity and low cost.It is suitable for the rapid detection of 41 sulfonamides and quinolones in liquid milk.

ultra-high performance liquid chromatography tandem triple quadrupole mass spectrometerC18rapid detectionsulfonamidesquinolones

黄珊、欧阳小艳、范云燕

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南宁市疾病预防控制中心,南宁 530023

超高效液相色谱串联三重四级杆质谱仪 C18 快速检测 磺胺 喹诺酮

2024

中国乳品工业
黑龙江乳品工业技术开发中心,中国乳制品工业协会

中国乳品工业

CSTPCD北大核心
影响因子:0.41
ISSN:1001-2230
年,卷(期):2024.52(11)