Isolation,identification and pathogenicity analysis of a strain of Serratia marcescens from a Chinese alligator
This study was aimed at identifying the cause of death of a young crocodile from the Chinese Alligator Manage-ment Center in Anhui Province.Disease material was collected from the necrotic brain tissue lesions of the young crocodile,the bacteria were isolated and identified,and pathogenicity was analyzed.The colony grew on blood agar plates as red,gram-nega-tive bacteria.Physiological and biochemical testsindicated that the bacteria were positive for glucose,mannitol,lactose,citrate and hydrogen sulfide.PCR amplification of the 16S rDNA gene,which is approximately 1 500 bp in size,and genetic evolu-tionary tree analysis were performed.The sequence was in the same branch as the reported TRD1 strain of Serratia marces-cens.Pathogenicity analysis indicated death of all mice within 36 hours,as well as severe pulmonary hemorrhage,liver enlarge-ment,spleen enlargement,and ascites.The pathogen isolated from the brain of the Chinese alligator that caused meningoen-cephalitis is Serratia marcescens,which has strong pathogenicity and resistance to a variety of antibacterial drugs.This study provides a reference for the diagnosis and treatment of Serratia marcescens infection in wild animals or humans.
Chinese alligatorSerratia marcescensidentificationpathogenicitydrug-resistance
万方数据
维普
