等温扩增技术在呼吸道病毒检测方面的研究进展
Research progress in the detection of respiratory viruses with isothermal amplification technology
杨森 1李桂梅 2滕新栋3
作者信息
- 1. 青岛农业大学,青岛 266109;青岛海关,青岛 266071
- 2. 青岛农业大学,青岛 266109
- 3. 青岛海关,青岛 266071
- 折叠
摘要
等温扩增技术是一种可在恒定温度条件下扩增核酸的技术,由于其具有仪器依赖性小、核酸扩增效率高、灵敏度高、操作简单、检测时间短等优点,十分适合在野外环境下或者疫病现场进行快速检测.本文介绍了等温扩增技术中的环介导等温扩增(loop-mediated isothermal amplification,LAMP)、重组酶聚合酶扩增(recombinase-polymerase amplification,RPA)、重组酶介导等温扩增(recombinase-aid amplifification,RAA)、依赖核酸序列的等温扩增(nucleic-acid sequence-based amplification,NASBA)、依赖解旋酶的等温扩增(helicase-dependent amplification,HDA)、交叉引物等温扩增(crossing-priming amplification,CPA)的原理、特点以及在呼吸道病毒检测方面的应用.
Abstract
Isothermal amplification technology can amplify nucleic acids under constant temperature conditions.Because of its advantages,including low instrument dependence,high nucleic acid amplification efficiency,high sensitivity,simple opera-tion,and short detection time,this method is highly suitable for rapid detection in outdoor environments or epidemic sites.This article focuses on loop mediated isothermal amplification(LAMP),recombinase polymerase amplification(RPA),recom-binase aided amplification(RAA),and nucleic acid sequence dependent isothermal amplification(NASBA)isothermal amplifi-cation technologies.The principles and characteristics of helicase dependent isothermal amplification(HDA)and cross primer i-sothermal amplification(CPA),and their applications in the detection of respiratory viruses,are reviewed.
关键词
等温扩增技术/检测/呼吸道病毒Key words
isothermal amplification technology/detection/respiratory virus引用本文复制引用
基金项目
山东省重点研发计划(重大科技创新工程)(2021CXGC011306)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据