Isothermal amplification technology can amplify nucleic acids under constant temperature conditions.Because of its advantages,including low instrument dependence,high nucleic acid amplification efficiency,high sensitivity,simple opera-tion,and short detection time,this method is highly suitable for rapid detection in outdoor environments or epidemic sites.This article focuses on loop mediated isothermal amplification(LAMP),recombinase polymerase amplification(RPA),recom-binase aided amplification(RAA),and nucleic acid sequence dependent isothermal amplification(NASBA)isothermal amplifi-cation technologies.The principles and characteristics of helicase dependent isothermal amplification(HDA)and cross primer i-sothermal amplification(CPA),and their applications in the detection of respiratory viruses,are reviewed.
国家科技期刊平台
NSTL
维普
万方数据
