中国人兽共患病学报2024,Vol.40Issue(4) :328-333.DOI:10.3969/j.issn.1002-2694.2024.00.049

甘肃省武威市部分地区蜱种调查及潜在病原体风险探讨

Investigation of tick species and potential pathogenic ricks in certain areas of Wuwei City,Gansu Province

李瑞山 贺真 袁祥 孙世伟 刘义文 张文凯 张琳 王玉华 陆振华 吉兆华 邵中军
中国人兽共患病学报2024,Vol.40Issue(4) :328-333.DOI:10.3969/j.issn.1002-2694.2024.00.049

甘肃省武威市部分地区蜱种调查及潜在病原体风险探讨

Investigation of tick species and potential pathogenic ricks in certain areas of Wuwei City,Gansu Province

李瑞山 1贺真 2袁祥 3孙世伟 2刘义文 3张文凯 2张琳 2王玉华 2陆振华 2吉兆华 2邵中军1
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作者信息

  • 1. 甘肃中医药大学公共卫生学院,兰州 730000;空军军医大学军事预防医学系军队防疫与流行病学教研室,特殊作业环境危害评估与防治教育部重点实验室,西安 710032
  • 2. 空军军医大学军事预防医学系军队防疫与流行病学教研室,特殊作业环境危害评估与防治教育部重点实验室,西安 710032
  • 3. 甘肃省武威市疾病预防控制中心,武威 733000
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摘要

目的 了解武威地区蜱类分布状况,丰富蜱种数据,为蜱媒疾病的预防提供依据.方法 于2021年、2022年的4-9月蜱活跃期在武威市山区采用布旗法和捡蜱法采集蜱;首先依据形态学特征进行鉴定和分类统计,其次根据线粒体16S rDNA和细胞色素C氧化酶亚基COXI基因进行PCR扩增和一代测序,获得序列在GenBank中比对,比对结果作为分子鉴定的依据.应用MEGA11.0软件采用邻近法构建系统进化树.结果 共采集蜱7 342只,隶属4属5种,分别为革蜱属的草原革蜱、璃眼蜱属的亚洲璃眼蜱、硬蜱属的犬硬蜱、血蜱属的长角血蜱和丹氏血蜱.系统进化树显示,采获的同一蜱种均位于同一进化分支上.结论 武威地区不同生境中共鉴定出5种蜱,不同生境中蜱的种类和数量不同,草原革蜱为该地区的优势蜱种.

Abstract

To understand the distribution of ticks in the Wuwei Region,enrich tick species data,and provide a basis for the prevention of tick-borne diseases,tick were collected using flagging and tick-picking methods during the highest activity period from April to September in 2021 and 2022 in the mountainous areas of Wuwei City.The ticks were identified based on morpho-logical and molecular biological characteristics,and characteristic sequences were obtained.A systematic evolutionary tree was constructed using the neighbor-joining method in MEGA 11.0 software.In total,7 342 ticks collected in Wuwei,which be-longed to 5 species from 4 genera with in the Ixodidae family,which included Dermacentor nuttalli,Hyalomma asiaticum,Ixodes canisuga,Haemaphysalis longicornis and Haema-physalis danieli.Ticks of the same species clustered together into the same branch of an evolutionary tree.In the Wuwei Re-gion,five common tick species are found across various habi-tats,with each habitat featuring different distributions of tick species and populations.The Dermacentor nuttalli is the dom-inant tick species in this area.

关键词

/蜱种鉴定/一代测序/遗传进化分析

Key words

tick/tick species identification/sanger se-quencing/genetic evolutionary analysis

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基金项目

国家自然科学基金(82273689)

陕西省重点研发计划(2024SF-YBXM-289)

武威市科技计划(WW190156)

出版年

2024
中国人兽共患病学报
中国微生物学会

中国人兽共患病学报

CSTPCD北大核心
影响因子:0.814
ISSN:1002-2694
参考文献量5
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