首页|广东地区人工驯养灵长动物的病毒组特征及猴痘病毒筛查

广东地区人工驯养灵长动物的病毒组特征及猴痘病毒筛查

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目的 明确广东地区灵长动物携带病毒群落的结构特征，并评估重要人兽共患病毒猴痘病毒(MPXV)通过人工驯养灵长动物传入我国的风险。方法 在广东省14个地级市的20个野生动物救护中心或动物园采集灵长动物样品，通过Illumina测序技术鉴定广东地区人工驯养灵长动物携带病毒组的结构特征;并通过荧光定量PCR方法，对MPXV进行检测，以确认MPXV经广东省人工驯养灵长动物传入的风险。结果 共收集灵长动物口咽拭子和粪便489份。高通量测序结果显示，广东地区人工驯养灵长动物粪便中的病毒组结构复杂且具有地区差异，其中丰度最高的是Alphaflexiviridae和Virga-viridae的成员，其次为Parvoviridae和Genomoviridae成员。荧光定量PCR结果显示，广东省内野生动物救护中心或动物园的灵长动物全部为MPXV阴性。结论 广东省人工驯养灵长动物携带病毒引起动物源性人兽共患病的风险极低，且MPXV通过广东省人工驯养灵长动物传入我国的风险几乎为零。
Virome characteristics and monkeypox virus screening of artificially domesticated primates in the Guangdong region
To clarify the structural characteristics of virus communities carried by primates in the Guangdong region,and evaluate the risk of the important zoonotic virus monkeypox virus(MPXV)being introduced into China through artificially do-mesticated primates,this study conducted metagenomic research on artificially domesticated primates and performed screening for MPXV.Primate samples were collected from 20 wildlife rescue centers or zoos in 14 prefecture level cities in Guangdong Province,and the structural characteristics of virus communities carried by artificially domesticated primates were identified through Illumina sequencing.Fluorescence quantitative PCR detection of MPXV excluded the risk of MPXV being introduced through artificially domesticated primates in Guangdong Prov-ince.A total of 489 oral and pharyngeal swabs and feces from primates were collected.High-throughput sequencing indicated that the viral group structure in the feces of artificially domesti-cated primates in the Guangdong region is complex and shows regional differences.Members of Alphaflexiviridae and Vir-gaviridae,followed by members of Parvoviridae and Genomo-viridae,had the highest abundance.Subsequently,fluorescence quantitative PCR results showed that all primates from wildlife rescue centers or zoos in Guangdong Province were MPXV neg-ative.This study provides the first description of the complex viral structure characteristics of artificially domesticated primates in the Guangdong region,and elucidates the differences in vi-ral communities among artificially domesticated primates in different regions.Our findings suggested that the risk of zoonotic diseases caused by artificially domesticated primates in Guangdong Province is extremely low,and the risk of MPXV being in-troduced into China through artificially domesticated primates in Guangdong Province is zero.

primatesviral metagenomemonkeypox viruszoonotic diseasesfluorescence quantitative PCR

李娜、任照文、张翩、袁子国、陈晓凡、廖明、王晓虎

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广东省农业科学院动物卫生研究所

广东省畜禽疫病防治研究重点实验室

农业农村部兽用药物与诊断技术广东科学观测实验站,广州 510640

华南农业大学兽医学院,广州 510640

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灵长动物 宏病毒组 猴痘病毒 人兽共患病 荧光定量PCR

广州市科技计划项目广东省自然资源事务专项资金-生态林业建设项目广东省农业科学院协同创新中心项目广东省现代农业产业技术体系创新团队项目广东省科技项目

2023E04J12564030132130299XT2022072022KJ1142021B1212050021

2024

10.3969/j.issn.1002-2694.2024.00.066

中国人兽共患病学报
中国微生物学会

中国人兽共患病学报

CSTPCD北大核心
影响因子：0.814
ISSN：1002-2694
年,卷(期)：2024.40(5)