摘要
目的 分析2021-2022年渝东北片区聚集性诺如病毒感染毒株遗传多样性,促进重点人群和场所诺如病毒感染防控.方法 采集聚集性诺如病毒感染事件中患者肛拭子及环境拭子,实时荧光定量PCR(qRT-PCR)检测诺如病毒核酸,逆转录PCR扩增病毒RNA聚合酶/衣壳蛋白片段并测序分析,诺如病毒分型工具网站(http://www.rivm.nl/mpf/norovirus/typingtool)确定基因型,运用MEGA-X软件按最大似然法构建种系发生树.结果 共调查渝东北片区4个区县11起聚集性诺如病毒感染事件,qRT-PCR检测55份样品,均为GⅡ基因群诺如病毒,经测序存在6种基因型.GⅡ.17[P17]基因型导致4起疫情,GⅡ.17[P17]和GⅡ.1[P16]基因型混合感染导致1起疫情,GⅡ.6[P7]及GⅡ.8[P8]基因型各引起2起疫情,GⅡ.2[P16]及GⅡ.3[P12]基因型分别导致1起疫情.种系发生分析显示55株GⅡ基因群毒株分布在5个基因簇.不同事件相同基因型的诺如病毒都位于同一个基因簇.不同事件不同基因型的诺如病毒各自聚集成簇,分属于不同的基因簇.结论 2021-2022年渝东北片区11起聚集性诺如病毒感染毒株涉及多种基因型,GⅡ.17[17]是引起聚集性感染事件的常见基因型,绝大多数事件由单一传染源引起,不同事件相同基因型的诺如病毒存在遗传相关性.
Abstract
Norovirus is the global leading cause of epidemic and endemic acute gastroenteritis in people of all ages.To inves-tigate the genetic diversity of GⅡ genogroup noroviruses linked to clustered infections in northeast Chongqing,we collected anal swabs or environmental smears from 11 norovirus outbreaks during 2021-2022.Norovirus RNA was detected by quantitative real-time PCR(qRT-PCR),and partial viral RdRp/capsid genes were amplified by reverse transcription PCR(RT-PCR)and sequenced.Among samples from 11 outbreaks in 4 districts and counties,55 strains of GⅡ genogroup norovirus were detected.Six genotypes were identified with an online norovirus genotyping tool(http://www.rivm.nl/mpf/norovirus/typingtool).Genotype GⅡ.17[P17]was associated with four outbreaks;the co-circulating GⅡ.17[P17]and GⅡ.1[P16]caused another out-break;GⅡ.6[P7]and GⅡ.8[P8]respectively were linked to two outbreaks;and GⅡ.3[P12]and GⅡ.2[P16]respectively ac-counted for one outbreak.Phylogenetic analysis also indicated that 55 GⅡ genogroup strains formed five clusters,with norovir-uses of identical genotypes from diverse events belonging to the same cluster,and that genetically distinct genotypes from di-verse events belonged to different clusters.Therefore,our results revealed that multiple genotypes associated with norovirus outbreaks were circulating in northeast Chongqing,and GⅡ.17[P17]was the predominant genotype linked to these out-breaks during 2021-2022.Most norovirus outbreak events were caused by single sources,and genetic relationships were demonstrated among noroviruses of identical genotypes from diverse events.
基金项目
重庆市教委科学技术研究项目(KJZD-K202202802)
重庆医药高等专科学校基金项目(YGZ2021107)
维普
万方数据