Multiple cross displacement amplification coupled with a gold nanoparticle-based lateral flow biosensor for detection of Listeria ivanovii
A method using multiple cross displacement amplification combined with a lateral flow biosensor to detect Liste-ria ivanovii was developed and evaluated in this study.The L.ivanovii-MCDA-LFB assay used a set of ten primers spanning ten distinct regions of smcl,and was performed at a constant temperature of 64 ℃ to evaluate its specificity,sensitivity and feasibility.The specificity of MCDA-LFB assay was 100％in L.ivanovii and non-L.ivanovii strains.The limit of detection of the MCDA-LFB assay in pure culture and in L.ivanovii artificial contaminated feces was 10 fg and 6.8× 102 CFU/g,respec-tively.The detection results of the L.ivanovii-MCDA-LFB assay were identical to those of traditional culture-based methods(ISO 11290-1)in 195 wild rat intestinal feces samples.Detection could be achieved by boiling the template with the second en-richment solution.Thus,our rapid L.ivanovii-MCDA-LFB method with favorable sensitivity and specificity may provide a useful tool for detecting L.ivanovii in food surveillance,and medical and laboratory research.
Listeria ivanoviimultiple cross displace-ment amplificationlateral flow biosensorsmcl genelimit of detection
万方数据
维普
