首页|sRNA 00085对单增李斯特菌环境适应性的影响

sRNA 00085对单增李斯特菌环境适应性的影响

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目的 探究单核细胞增生李斯特菌生物被膜相关非编码小RNA 00085(sRNA00085)对该菌生存及环境适应能力的调控作用.方法 采用同源重组技术构建sRNA 00085靶基因的缺失株△sRNA 00085及回补株C△sRNA00085,并对比标准株、缺失株及回补株生长能力、生物被膜形成能力及抗生素敏感性方面的差异.结果 与标准株相比,△sRNA00085株的生物被膜形成能力及对青霉素、哌拉西林、强力霉素、四环素、万古霉素、复方新诺明的敏感性显著降低,其中仅对四环素的最低抑菌浓度(MIC)表现出显著降低,而C△sRNA00085株在这些特性上与标准株无显著差异.进一步检测发现,敲除sRNA 00085后,单核细胞增生李斯特菌四环素耐药相关基因tetS转录水平显著升高,但对tetA的转录无影响.结论 sRNA00085通过调控单核细胞增生李斯特菌的生物被膜形成能力及多种抗生素耐药性,从而影响其环境适应性.
Effects of sRNA 00085 on the environmental fitness of Listeria monocytogenes
The purpose of this study was to investigate the regulatory effects of biofilm associated non-coding small RNA(sRNA)00085 on the survival and environmental fitness of Listeria monocytogenes.Homologous recombination technology was used to construct a deletion mutant strain(△sRNA 00085)and a complementary strain(C △sRNA 00085)of the sRNA00085 target gene.The differences in biological characteristics were compared among the standard strain,△sRNA 00085,and C△sRNA 00085.The deletion of sRNA00085 led to a significant decrease in biofilm formation capacity and sensitivity to several antibiotics,including penicillin,piperacillin,doxycycline,tetracycline,vancomycin,and cotrimoxazole.However,only the minimum inhibitory concentration(MIC)of tetracycline exhibited a significant decrease in △sRNA00085.Meanwhile,the decreased biofilm formation and antibiotic resistance of the sRNA00085 mutant were restored in the C△sRNA00085 strain.Furthermore,we investigated the transcription levels of tetracycline resistance-related genes in L.monocytogenes.Down-regu-lated transcription of the tetS gene but no significant difference in transcription of the tetA gene were observed in △sRNA 00085 compared with the standard strain and C△sRNA00085.Moreover,the elimination of sRNA00085 did not affect bacterial growth ability or sensitivity to disinfectants.These findings highlight that sRNA00085 plays an important role in the environ-mental adaptability of L.monocytogenes by affecting bacterial biofilm formation and resistance.

Listeria monocytogenesnon-coding small RNAbiofilmresistanceenvironmental fitness

邓静、芝吉、范子秋、赵学慧、宋雅丽、苟惠天、魏衍全、曹青

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甘肃农业大学动物医学院,兰州 730070

单核细胞增生李斯特菌 非编码小RNA 生物被膜 耐药性 环境适应性

甘肃农业大学公招博士科研启动金基金项目国家自然科学基金项目国家自然科学基金项目甘肃省技术创新引导计划项目甘肃省自然科学基金青年项目张家川揭榜挂帅项目

GAU-KYQD-2022-36320608223156070023CXNA001621JR7RA482ZC-STK-2023A-030

2024

10.3969/j.issn.1002-2694.2024.00.097

中国人兽共患病学报
中国微生物学会

中国人兽共患病学报

CSTPCD北大核心
影响因子：0.814
ISSN：1002-2694
年,卷(期)：2024.40(7)