首页|基于groEL基因检测云南家鼠鼠疫疫源地鼠类动物感染米库尔新埃立克体情况

基于groEL基因检测云南家鼠鼠疫疫源地鼠类动物感染米库尔新埃立克体情况

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目的 调查云南家鼠鼠疫疫源地鼠类动物感染米库尔新埃立克体情况.方法 以云南省梁河县、芒市和弥勒市鼠疫疫源地为采样点,使用夹夜法捕捉鼠类动物,并收集脾脏样本.采用巢式PCR技术检测脾脏样本中米库尔新埃立克体特异性groEL基因,并对阳性产物进行Sanger双向序列测定.应用x2检验或Fisher确切概率法分析不同疫源地、生境、鼠种、性别间鼠类动物感染米库尔新埃立克体的情况.结果 检测656份鼠类动物样本,在黄胸鼠、斯氏家鼠、北社鼠和青毛巨鼠上检出米库尔新埃立克体阳性12份,阳性率为1.83%.不同疫源地、生境、鼠种、性别间鼠类动物感染米库尔新埃立克体的阳性率均无统计学差异(P>0.05).基于groEL基因的遗传进化分析,12份阳性样本核酸序列内部的同源性在99.5%~100%,米库尔新埃立克体核酸序列处于同一分支,为Cluster Ⅳ型.结论 云南家鼠鼠疫疫源地4种鼠类动物存在米库尔新埃立克体低度感染.
Detection of Neoehrlichia mikurensis in rodents on the basis of the groEL gene in Yunnan commensal rodent plague foci
The purpose of this study was to understand the prevalence of Neoehrlichia mikurensis in rodents in Yunnan commensal rodent plague foci.Lianghe Country,Mangshi City,and Mile City in Yunnan Province were chosen as sampling sites,where rodents were captured with dead-traps.The N.mikurensis groEL gene in rodent spleen samples was detected with nested PCR,and the positive products were sequenced with Sanger bidirectional assays.The infection rate of N.mikurensis a-mong plague foci,habitats,species,and sexes was compared with Chi-square tests or Fisher's exact probability method.Of 656 rodent spleen samples,12 N.mikurensis positive samples were detected in R.tanezumi,R.sladeni,N.confucianus,and B.bowersi.The positivity rate was 1.83%.No significant difference in the N.mikurensis positivity rate was observed a-mong plague foci,habitats,species,and sexes(P>0.05).Genetic evolution analysis of the groEL gene indicated that the se-quence similarity of nucleic acid sequences in 12 positive samples was 99.5%-100%,and the nucleic acid sequences of N.mikurensis were in the same branch,belonging to cluster Ⅳ.Thus,four species of rodents were found to have low frequency infection with N.mikurensis in Yunnan commensal rodent plague foci.

Neoehrlichia mikurensisgroEL genepositive raterodentscommensal rodent plague foci

韦蓉、李紫薇、罗云燕、汪娜、刘淑清、李进春、卢江丽、尹家祥

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大理大学公共卫生学院,大理 671000

米库尔新埃立克体 groEL基因 阳性率 鼠类 家鼠鼠疫疫源地

国家自然科学基金云南省兴滇英才支持计划云南省高校自然疫源性疾病流行病学科技创新团队

81860565YNWR-MY-2019-008云教发[2020]102号

2024

中国人兽共患病学报
中国微生物学会

中国人兽共患病学报

CSTPCD北大核心
影响因子:0.814
ISSN:1002-2694
年,卷(期):2024.40(7)