Investigation and analysis of a familial cluster of Brucella canis in dogs
This study was aimed at investigating and analyzing a familial cluster of Brucella canis among dogs in Chaoyang District,Beijing.The goal was to describe the transmission modes and risks of this disease among urban pet dogs,to provide scientific evidence for its detection and prevention.Blood,urine,conjunctival swabs,oral and nasal swabs,reproductive tract swabs,and rectal swabs were collected from all dogs in an affected household.These samples underwent serological testing for brucellosis,qPCR detection,and bacterial culture.The isolated strains were identified with Bruce-Ladder PCR.Additionally,the dogs'medical and contact histories were reviewed and analyzed.Four dogs in the household were found to be infected with Brucella canis.One dog was found to be infected with Brucella canis,on the basis of positive qPCR or bacterial culture tests in urine,conjunctival swabs,and blood.The other three infected dogs had a history of roaming.No mating behavior was observed among the dogs in this study.This outbreak of Brucella canis probably spread through non-reproductive routes,with urine and ocular secretions from the infected dogs being potential significant sources of transmission.