摘要
目的 对北京朝阳区一起宠物犬间犬种布鲁氏菌病家庭聚集性疫情进行调查分析,讨论城市宠物犬间该病传播方式及传播风险,为其检测及防控提供科学依据.方法 采集疫情所在宠物家庭的所有犬只的血液、尿液、眼结膜拭子、口鼻拭子、生殖道拭子和肛拭子样本,进行布病血清学、qPCR检测和细菌分离培养,并应用Bruce-Ladder PCR对分离菌株进行种型鉴定;了解犬只既往病史及接触史,对结果进行描述和分析.结果 该家庭中存在4只犬种布鲁氏菌感染犬,1只患犬尿液、眼结膜拭子和血液经qPCR或细菌培养检测为阳性,经鉴定为犬种布鲁氏菌,另3只感染犬存在流浪史.本研究中所有犬间无交配行为.结论 本起宠物犬间犬种布鲁氏菌病疫情可能通过非生殖途径在犬间传播,患犬尿液和眼分泌物可能是重要的传染源.
Abstract
This study was aimed at investigating and analyzing a familial cluster of Brucella canis among dogs in Chaoyang District,Beijing.The goal was to describe the transmission modes and risks of this disease among urban pet dogs,to provide scientific evidence for its detection and prevention.Blood,urine,conjunctival swabs,oral and nasal swabs,reproductive tract swabs,and rectal swabs were collected from all dogs in an affected household.These samples underwent serological testing for brucellosis,qPCR detection,and bacterial culture.The isolated strains were identified with Bruce-Ladder PCR.Additionally,the dogs'medical and contact histories were reviewed and analyzed.Four dogs in the household were found to be infected with Brucella canis.One dog was found to be infected with Brucella canis,on the basis of positive qPCR or bacterial culture tests in urine,conjunctival swabs,and blood.The other three infected dogs had a history of roaming.No mating behavior was observed among the dogs in this study.This outbreak of Brucella canis probably spread through non-reproductive routes,with urine and ocular secretions from the infected dogs being potential significant sources of transmission.
维普
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