Isolation,Identification and Prevention of Main Pathogenic Bacteria of Dairy Goat Mastitis
[Objective]To isolate and identify the main bacteria causing mastitis in a dairy goat farm in Linyi area of Shandong Province,and to explore its drug sensitivity.[Methods]In this experiment,milk samples of dairy goats with mastitis were collected from a dairy goat farm in Linyi area,Shandong Province,and pathogens were isolated by bacterial culture medium.PCR amplification technology was used for sequencing and database comparison to identify bacteria.The resistance of Escherichia coli and Staphylococcus aureus to antibiotics commonly used in clinical practice was determined by Kirby-Bauer disk diffusion method.[Result]A total of 109 pathogenic bacteria were isolated and identified,among which Escherichia coli and Staphylococcus aureus accounted for the most,41.28%and 28.44%,respectively.The results showed that Escherichia coli and Staphylococcus aureus had high resistance to penicillin G and clindamycin,but were more sensitive to quinolones and cephalosporins.Among them,Staphylococcus aureus had the highest sensitivity to cefotaxime sodium(97.0%),while Escherichia coli had the highest sensitivity to ofloxacin(73.8%).It is suggested that these two antibiotic drugs can be used to treat mastitis of dairy goats in this farm.[Conclusion]The main bacterial pathogens of mastitis in dairy goats in this farm are Staphylococcus aureus and Escherichia coli,which are the most sensitive to cefotaxime sodium and ofloxacin respectively,and are recommended for clinical treatment.
dairy goatmastitispathogenic bacteriaisolation and identificationprevention and control measures
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