酶联免疫吸附和胶体金技术对黄曲霉毒素M1检测的结合验证
Combined Validation of Aflatoxin M1 Detection by Enzyme-linked Immunoassay Techniques and Colloidal Gold Techniques
乔宽 1王路阳 1王艳飞 1张云鲜 1时长旭1
作者信息
- 1. 蒙牛乳业(焦作)有限公司,河南焦作 454100
- 折叠
摘要
[目的]为准确完成生牛乳中黄曲霉毒素M1检测.[方法]对比分析酶标仪、胶体金读数仪检测生牛乳中黄曲霉毒素M1的处理过程和检测数据.[结果]发现2 种方法检验操作过程各有优劣,检测结果能满足不同需求.酶标仪可用于半定量检测,并进行精准检测和半定量复核.胶体金读数仪适合快速定性筛查.[结论]2 种方法可配合使用,相互补充,灵活运用设备优势,高效完成检测需求.
Abstract
[Objective]To accurately complete the detection of aflatoxin M1 in raw milk.[Method]This paper used a microplate reader and a colloidal gold reader to detect the treatment process and detection data of aflatoxin M1 in raw milk for comparative analysis.[Result]We discovered the advantages and disadvantages of two methods for verifying the operation process,and the test results could meet different needs.The microplate reader could be used for semi-quantitative detection,and the microplate reader could be used for accurate detection and semi-quantitative review.The colloidal gold reader was suitable for rapid qualitative screening.[Conclusion]The two methods could be used together,complement each other,flexibly use the advantages of various equipments,and could efficiently complete various testing requirements.
关键词
酶标仪/胶体金读数仪/黄曲霉毒素M1/生牛乳Key words
colloidal gold reader/microplate reader/aflatoxin M1/raw milk引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据