酸奶中乙酰磺胺酸钾、苯甲酸、山梨酸和糖精钠的检测方法
Detection Method for Acesulfame Potassium,Benzoic Acid,Sorbic Acid,and Saccharin Sodium in Yogurt
凌玲 1周雨 1袁媛 1安娜1
作者信息
- 1. 北京市石景山区疾病预防控制中心理化科,北京 100043
- 折叠
摘要
[目的]建立使用高效液相色谱仪同时检测酸奶中的乙酰磺胺酸钾等4 种食品添加剂的方法.[方法]酸奶样品经过蛋白沉淀剂沉淀蛋白后高速离心,经0.22 µm微孔滤膜过滤后上机测定.仪器使用C18色谱柱,流动相采用甲醇与0.02 mol/L乙酸铵溶液(不需调节pH值),检测波长为227 nm.[结果]4 种添加剂线性范围1.00～50.0 mg/L,相关系数均在0.999 9以上,回收率91.2%～104.8%.[结论]该方法操作简单、快速,可用于同时检测酸奶中的乙酰磺胺酸钾、苯甲酸、山梨酸和糖精钠4种食品添加剂.
Abstract
[Objective]To establish a method for the simultaneous detection of four food additives,including acesulfame potassium,in yogurt using high-performance liquid chromatography.[Method]Yogurt samples were precipitated with a protein precipitant and then centrifuged at high speed.The supernatant was filtered through a 0.22 µm pore size filter membrane before being measured by the instrument.A C18 chromatographic column was used,with methanol and a 0.02 mol/L ammonium acetate solution(without pH adjustment)as the mobile phase,and the detection wavelength was set at 227 nm.[Result]The linear range of the four food additives was 1.00-50.0 mg/L,with correlation coefficients above 0.9999,and recovery rates between 91.2%and 104.8%.[Conclusion]This method is simple,rapid,and can be used for the simultaneous detection of acesulfame potassium,benzoic acid,sorbic acid,and sodium saccharin in yogurt.
关键词
高效液相色谱法/食品添加剂/同时检测Key words
high-performance liquid chromatography/food additives/simultaneous detection引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据