Detection Method for Acesulfame Potassium,Benzoic Acid,Sorbic Acid,and Saccharin Sodium in Yogurt
[Objective]To establish a method for the simultaneous detection of four food additives,including acesulfame potassium,in yogurt using high-performance liquid chromatography.[Method]Yogurt samples were precipitated with a protein precipitant and then centrifuged at high speed.The supernatant was filtered through a 0.22 µm pore size filter membrane before being measured by the instrument.A C18 chromatographic column was used,with methanol and a 0.02 mol/L ammonium acetate solution(without pH adjustment)as the mobile phase,and the detection wavelength was set at 227 nm.[Result]The linear range of the four food additives was 1.00-50.0 mg/L,with correlation coefficients above 0.9999,and recovery rates between 91.2%and 104.8%.[Conclusion]This method is simple,rapid,and can be used for the simultaneous detection of acesulfame potassium,benzoic acid,sorbic acid,and sodium saccharin in yogurt.
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