酸奶中1株季也蒙假丝酵母的分离鉴定
Isolation and Identification of One Plant of Meyerozyma guilliermondii in Yogurt
马静 1骆敏 2宋艳梅 2谭莲英 2范光彩 2周欢 2钱成林 2夏忠悦3
作者信息
- 1. 新希望乳业股份有限公司,四川成都 610023;国家乳业技术创新中心,呼和浩特 010020
- 2. 新希望乳业股份有限公司,四川成都 610023
- 3. 新希望乳业股份有限公司,四川成都 610023;乳品营养与功能四川省重点实验室,四川成都 610023;国家乳业技术创新中心,呼和浩特 010020
- 折叠
摘要
[目的]鉴定分析酸奶中检出酵母,快速分析查找来源,控制产品质量,预防质量问题产生.[方法]以胀包酸奶为对象,采用孟加拉红培养基测定产品中酵母菌,分离纯化后,经菌落形态和细胞形态观察确认为酵母菌,分别采用生化鉴定及真菌种属鉴定(ITS)分离菌株.[结果]分离菌株为单一菌株,其为季也蒙假丝酵母.[结论]为生产中酸奶污染菌株的分析提供理论指导及参考方向.
Abstract
[Objective]Identify and analyze the yeast detected in yogurt,quickly analyze to find the source,control product quality and prevent product quality problems.[Method]The yeast from bulged yogurt was determined by Rose Bengal Agar.After isolation and purification,the isolated strain was verified by observation of bacterial colony and cellular morphology.It was identified by physiological and biochemical identification and ITS identification respectively.[Result]The isolated strains were a single strain,which was Meyerozyma guilliermondii.[Conclusion]This study provided theoretical guidance and reference direction for the analysis of contaminated strains of yogurt in production.
关键词
酸奶/胀包/生化鉴定/ITS/季也蒙假丝酵母Key words
yogurt/bulged/biochemical identification/ITS/Meyerozyma guilliermondii引用本文复制引用
基金项目
四川省科技计划资助(2022ZHCG0129)
四川省科技计划(2023NSFSC0018)
出版年
2024