Determination of 1,3-dioleoyl-2-palmitoylglycerol in Infant Formula Milk Powder
[Objective]Establish a gas chromatography method to determinate the content of 1,3-dioleoyl-2-palmitoylglycerol in infant formula.[Method]The infant formula samples were dissolved in warm water,saponified with ammonia,extracted oil with ethyl ether and petroleum ether,dissolved in n-hexane,separated by DB-1HT capillary column,determined the content of 1,3-dioleoyl-2-palmitoylglycerol by comparison with standard triglyceride solution,and quantified by internal standard method.[Result]The quantitative limit of method was 0.008 mg/100 g with good linear range.The recoveries of low,medium and high standard addition levels were 81.1%～98.4%,and the relative standard deviations were 2.89%～7.88%.RSD of 1,3-dioleoyl-2-palmitoylglycerol in the matrix samples of infant formula sheep milk powder and cow milk powder was determined to be less than 3.03%.[Conclusion]This method has the advantages of simple operation,short analysis period,high sensitivity and good repeatability.It is suitable for the determination of 1,3-dioleoyl-2-palmitoylglycerol content in infant formula cow milk powder and sheep milk powder,and has ideal quantitative effect and application prospect.
gas chromatography1,3-dioleoyl-2-palmitoylglycerolinfant formula milk powder
国家科技期刊平台
NSTL
万方数据
维普
