婴幼儿配方奶粉中1,3-二油酸-2-棕榈酸甘油三酯的测定
Determination of 1,3-dioleoyl-2-palmitoylglycerol in Infant Formula Milk Powder
徐旖 1陈小丽 1王潇 2陈双 1吕晓蓉 1汤陈龙 1孟奇1
作者信息
- 1. 浙江公正检验中心有限公司,浙江杭州 310009
- 2. 浙江公正检验中心有限公司,浙江杭州 310009;浙江省食品安全重点实验室,浙江杭州 310009
- 折叠
摘要
[目的]建立气相色谱检测婴幼儿配方奶粉中1,3-二油酸-2-棕榈酸甘油三酯的含量.[方法]婴幼儿配方奶粉样品经温水溶解、氨水皂化、乙醚石油醚提取油脂,正己烷溶解,采用Agilent DB-1HT毛细管柱分离,通过与标准甘油三酯溶液对比确定出峰位置,采用内标法定量.[结果]方法定量限为0.008 mg/100 g,线性范围良好.低、中、高3 个标准添加水平的回收率为81.1%～98.4%,相对标准偏差在2.89%～7.88%.对婴幼儿配方羊奶粉、牛奶粉基质样品中1,3-二油酸-2-棕榈酸甘油三酯的含量测定RSD≤3.03%.[结论]该方法操作过程简单、分析周期短、灵敏度高、重复性好,适用于测定婴幼儿配方牛奶粉、婴幼儿配方羊奶粉中1,3-二油酸-2-棕榈酸甘油三酯的含量,具有理想的定量效果及应用前景.
Abstract
[Objective]Establish a gas chromatography method to determinate the content of 1,3-dioleoyl-2-palmitoylglycerol in infant formula.[Method]The infant formula samples were dissolved in warm water,saponified with ammonia,extracted oil with ethyl ether and petroleum ether,dissolved in n-hexane,separated by DB-1HT capillary column,determined the content of 1,3-dioleoyl-2-palmitoylglycerol by comparison with standard triglyceride solution,and quantified by internal standard method.[Result]The quantitative limit of method was 0.008 mg/100 g with good linear range.The recoveries of low,medium and high standard addition levels were 81.1%～98.4%,and the relative standard deviations were 2.89%～7.88%.RSD of 1,3-dioleoyl-2-palmitoylglycerol in the matrix samples of infant formula sheep milk powder and cow milk powder was determined to be less than 3.03%.[Conclusion]This method has the advantages of simple operation,short analysis period,high sensitivity and good repeatability.It is suitable for the determination of 1,3-dioleoyl-2-palmitoylglycerol content in infant formula cow milk powder and sheep milk powder,and has ideal quantitative effect and application prospect.
关键词
气相色谱/1,3-二油酸-2-棕榈酸甘油三酯/婴幼儿配方奶粉Key words
gas chromatography/1,3-dioleoyl-2-palmitoylglycerol/infant formula milk powder引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据