A2β-酪蛋白牛奶和普通牛奶的体外消化特性和抗氧化能力评价
Evaluation of In Vitro Digestive Characteristics and Antioxidant Capacity of A2β-casein Milk and Regular Milk
窦佩佩 1张慧娟 1卢涵 2袁庆彬 3王世杰 4罗永康1
作者信息
- 1. 中国农业大学食品科学与营养工程学院,北京 100083
- 2. 河北科技大学食品与生物学院,河北石家庄 050018
- 3. 石家庄君乐宝乳业有限公司,河北石家庄 050221
- 4. 河北科技大学食品与生物学院,河北石家庄 050018;石家庄君乐宝乳业有限公司,河北石家庄 050221
- 折叠
摘要
[目的]研究体外模拟胃肠消化对A2β-酪蛋白牛奶和普通牛奶的分子量分布及抗氧化能力的影响.[方法]以巴氏杀菌A2β-酪蛋白牛奶、超高温灭菌A2β-酪蛋白牛奶、巴氏杀菌普通牛奶、超高温灭菌牛奶为原料,通过INFOGEST 2.0对牛奶进行体外模拟胃肠消化,采用SDS-PAGE、高效液相色谱仪对体外模拟胃肠消化前后的牛奶样品进行分子量分析,并对体外模拟胃肠消化后各样品的抗氧化能力进行测定.[结果]在体外模拟胃肠消化后,4 种牛奶的分子量主要分布于<1 000 Da组分,A2β-酪蛋白牛奶小于500 Da组分的比例显著低于普通牛奶.超高温灭菌普通牛奶、超高温灭菌A2β-酪蛋白牛奶表现出较强的铁离子还原能力和1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除能力.[结论]本研究为各类牛奶产品的加工和功能特性提供基础数据.
Abstract
[Objective] This study aims to investigate the effects of in vitro simulated gastrointestinal digestion on the molecular weight distribution and antioxidant activities of A2β-casein milk and regular milk. [Method] The pasteurized A2β-casein milk, ultra-high temperature sterilized A2β-casein milk, pasteurized regular milk, and ultrahigh temperature sterilized regular milk were used as materials. The milks were subjected to in vitro simulated gastrointestinal digestion using the INFOGEST 2.0 system. Molecular weight analysis of the milk samples before and after digestion was performed using SDS-PAGE and high-performance liquid chromatography, and the antioxidant activities of the samples post-digestion were measured. [Result] After in vitro simulated gastrointestinal digestion, the molecular weight of the four types of milk was mainly distributed in the <1 000 Da, with the proportion of components <500 Da in A2β-casein milk significantly lower than in regular milk. Ultra-high temperature sterilized regular milk and ultra-high temperature sterilized A2β-casein milk showed stronger ferric ion reducing antioxidant power and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. [Conclusion] This study provided basic data for the processing and functional characteristics of various milk products.
关键词
A2β-酪蛋白/普通牛奶/体外模拟胃肠消化/分子量/抗氧化能力Key words
A2β-casein milk/regular milk/in vitro simulated gastrointestinal digestion/molecular weight/antioxidant activity引用本文复制引用
基金项目
京津冀协同创新共同体建设专项(22342901D)
石家庄市引进国外智力项目(20230025)
石家庄后补助奖励专项资金(226790217H)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据