[目的]建立山羊痘的病理学、血清学及分子生物学诊断方法.[方法]选择山东省临沂市某大型养殖场感染山羊痘病毒的50 例奶山羊作为观察组,同期未感染的健康奶山羊50 例作为对照组,采集血液及相关病变组织材料进行病理学、血清学和分子生物学检测.[结果]病理剖检显示,山羊痘病的奶山羊皮肤、口腔、鼻腔、喉头、气管黏膜及肝脏、肾脏、肺脏及胃组织等多处出现较多典型疹痘.琼脂糖扩散试验显示,山羊痘沉淀抗原与阳性血清发生反应,琼脂糖凝胶孔出现白色沉淀线;健康奶山羊对照抗原则没有任何沉淀线出现.高敏荧光检测显示,感染山羊痘的奶山羊血清抗体效价显著高于健康奶山羊(96.24±5.73 vs 4.01±0.26,P<0.05).PCR鉴定结果显示,鼻拭子样本、眼拭子样本、肺组织样本和阳性对照样本可扩增出455 bp特异性电泳条带.[结论]成功建立一种针对山东省临沂地区奶山羊感染山羊痘病毒的诊断方法,为后期该地区各类型养殖场山羊痘病控制和临床防治提供科学的参考依据.
Pathological,Serological and Molecular Biological Diagnosis of Goat Pox
[Objective]To establish pathological,serological and molecular biological diagnostic methods for goat pox.[Method]50 dairy goats infected with goat pox virus in a large farm in Linyi City,Shandong Province were selected as the observation group,and 50 healthy dairy goats that uninfected during the same period were selected as the control group.Blood and related pathological tissue materials were collected for pathological,serological and molecular biological detection.[Result]Pathological examination showed that the skin,oral cavity,nasal cavity,larynx,tracheal mucosa,liver,kidney,lung and stomach tissues of dairy goats with goat pox disease showed many typical acne.Agarose diffusion test showed that goat pox precipitated antigen reacted with positive serum and white precipitation lines appeared in agarose gel pores,and there were no precipitation line was observed in healthy dairy goats control group.High sensitivity fluorescence detection showed that the serum antibody titer of dairy goats infected with goat pox was significantly higher than that of healthy dairy goats(96.24±5.73 vs 4.01±0.26,P<0.05),and the difference was statistically significant.PCR identification results showed that 455 bp specific electrophoresis bands could be amplified from nose swab,eye swab,lung tissue and positive control samples.[Conclusion]This study has successfully established a diagnostic method for goat pox virus infection in dairy goats in Linyi area,Shandong Province,and provided scientific reference for the control and clinical prevention of goat pox disease in various types in this area.
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