Stability of Bovine and Goat Milk Fat Globule Membranes and Differences in Untargeted Lipidomics
Chinese Holstein cows and dairy goats in Guanzhong region of China were used as research subjects to analyze milk fat globule membrane lipids based on non-targeted lipidomics technology to screen for membrane differential lipids.The results showed that 3 004 and 2 857 lipids were detected in cow and goat milk MFGM,respectively.Phospholipids,glycolipids,sterols and sphingolipids accounted for 75.94%,8.29%,3.82%and 3.76%of the total content of lipids other than glycerides in cow's milk as compared to 66.98%,12.42%,4.27%,and 3.57%in goat's milk,respectively.In addition,1 496 significantly differentiated lipids were present on bovine and sheep MFGM,in which glyceride molecules such as TG(17︰0/10︰0/22︰6),TG(16︰0e/8︰0/18︰1),TG(8︰0/10︰4/14︰2),TG(14︰0/14︰0/22︰6),and DG(15︰0/16︰0)were associated with the same lipid profile as CL(87︰12),PE(41︰2e),SM(t39︰7),CL(66︰1),MePC(29︰2e),MFGM lipid molecules such as PE(16︰0/16︰1)and PI(18︰1/20︰3)can be used for categorization and identification of cow's and sheep's milk as well as differentiation of the targets of the assay,which can provide the theoretical basis of the detection of cow's and sheep's milk lipids.
breast's and goat's milklipid globule membrane lipidfatty acidlipidomicsvariance analysis