PCR Diagnosis of Mixed Infection of Bovine Viral Diarrhea Virus and Coronavirus
[Objective]To establish a PCR diagnostic method for mixed infection of Bovine Viral Diarrhea Virus(BVDV)and Bovine Coronavirus(BCoV).[Method]98 cows infected with BVDV and BCoV in a dairy farm in Shandong were selected as the research subjects.Their feces and tissues were collected and virus genomic RNA was extracted.After reverse transcription,primers were designed for PCR detection,and the detection status,specificity and repeatability were analyzed.[Result]40 cases were infected with BVDV and BCoV(40.82%),including 33 fecal samples(40.27%)and 7 tissue samples accounted for 7 cases(43.75%).There were 27 cases(27.55%)of BVDV single infection,including 21 fecal samples(25.61%)and 6 tissue samples(37.50%).There were 32 cases(32.65%)of BCoV single infection,including 28 fecal samples(34.15%)and 4 tissue samples(25.00%).[Conclusion]In this study,the PCR diagnostic method for the mixed infection of BVDV and BCoV was accurate,with good specificity and repeatability,which provided a reference for the correct diagnosis of these two viruses in clinical practice and laid a foundation for precise and scientific prevention and control in the future.
国家科技期刊平台
NSTL
万方数据
