Method Validation Study for the Determination of Aflatoxin M1 Content in Milk by High Performance Liquid Chromatography
According to GB 5009.24—2016 National Standard for Food Safety,Determination of Aflatoxins M1 Group in Food,the second method was extracted by methanol-water solution,a method for the determination of aflatoxins M1 in milk by high performance liquid chromatography with high performance liquid chromatography was developed,the detection limit,quantitative limit,linear range,accuracy and repeatability precision were tested.The results showed that the aflatoxins M1 in milk had a good linearity in the range of 0.00250～2.02 ng/mL,and the correlation coefficient was larger than that of the 0.999.The detection limit of the instrument was 0.00359 μg/kg,and that of the method was 0.00180 μg/kg,the quantitative limit of the instrument was 0.0108 μg/kg,the quantitative limit of the method was 0.00540 μg/kg,the recoveries were 95.80％～103.37％,the repeatability precision was 0.46％～1.66％,all accord GB 5009.295—2023 The Requirements of National Standard for Food Safety General Rules for Verification of Chemical Analysis Methods.
high performance liquid chromatographymilkaflatoxins M1method validation
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